概述

  • 产品名称Anti-JunB抗体
    参阅全部 JunB 一抗
  • 描述
    兔多克隆抗体to JunB
  • 特异性Antibody detects endogenous levels of JunB protein around Serine 259.
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from Human JunB around the phosphorylation site of Serine 259.

  • 阳性对照
    • Extracts of Hela cells and breast carcinoma tissue. Hek293 clls.

性能

应用

Our Abpromise guarantee covers the use of ab31421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

靶标

Anti-JunB antibody 图像

  • ICC/IF image of ab31421 stained Hek293 cells (ab7902). The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31421, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-JunB antibody (ab31421) at 1/500 dilution

    Lane 1 : Extracts of Hela cells.
    Lane 2 : Extracts of Hela cells. Antibody pre-incubated with synthesized peptide.


    Predicted band size : 35 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
  • ab31421 at a 1:50-1:100 dilution, staining JunB in human breast carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.

    Left image : Un-treated.

    Right image : Antibody pre-incubated with synthesized peptide.

Anti-JunB antibody (ab31421)参考文献

This product has been referenced in:
  • Marban C  et al. Interplay between the HTLV-2 Tax and APH-2 proteins in the regulation of the AP-1 pathway. Retrovirology 9:98 (2012). IP ; Human . Read more (PubMed: 23206352) »
  • Raffaello A  et al. JunB transcription factor maintains skeletal muscle mass and promotes hypertrophy. J Cell Biol 191:101-13 (2010). WB ; Mouse . Read more (PubMed: 20921137) »

See all 4 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Other - EMSA
Sample Human Cell lysate - nuclear (renal cancer cells)
Specification renal cancer cells
Total protein in input 3 µg
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提交于 Dec 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"