Anti-JNK2抗体[EP1595Y] (ab76125)
- 数据表
- 具体的参考文献 (12)
- 实验方案
概述
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产品名称Anti-JNK2抗体[EP1595Y]
参阅全部 JNK2 一抗 -
描述兔单克隆抗体[EP1595Y] to JNK2
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宿主Rabbit
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经测试应用适用于: WB, IP, IHC-P, Flow Cytmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
A synthetic peptide corresponding to residues near the C terminus of human JNK2.
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阳性对照
- HeLa cell lysate; human breast carcinoma tissue.
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常规说明
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EP1595Y
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同种型IgG
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研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Positive Controls
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Related Products
应用
Our Abpromise guarantee covers the use of ab76125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 48 kDa. | |
| IP | Use a concentration of 5 µg/ml. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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靶标
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功能Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. -
序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
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翻译后修饰Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Autophosphorylated in vitro.
- Information by UniProt
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数据库链接
- Entrez Gene: 5601 Human
- Entrez Gene: 26420 Mouse
- Entrez Gene: 50658 Rat
- Omim: 602896 Human
- SwissProt: P45984 Human
- SwissProt: Q9WTU6 Mouse
- SwissProt: P49186 Rat
- Unigene: 484371 Human
see all -
别名
- c Jun kinase 2 antibody
- C Jun N terminal kinase 2 antibody
- c-Jun N-terminal kinase 2 antibody
see all
图片
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![Western blot - Anti-JNK2 antibody [EP1595Y] (ab76125)](http://www.abcam.cn/ps/products/76/ab76125/Images/ab76125-256189-ab76125KOWB.jpg)
Western blot - Anti-JNK2 antibody [EP1595Y] (ab76125)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: JNK2 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76125 observed at 54 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76125 was shown to specifically react with JNK2 when JNK2 knockout samples were used. Wild-type and JNK2 knockout samples were subjected to SDS-PAGE. ab76125 and ab8245 (loading control to GAPDH) were diluted 1/2500 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Immunoprecipitation - Anti-JNK2 antibody [EP1595Y] (ab76125)](http://www.abcam.cn/ps/products/76/ab76125/Images/ab76125-171832-IPV018ab7612520mMod.jpg)
Immunoprecipitation - Anti-JNK2 antibody [EP1595Y] (ab76125)JNK2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit monoclonal to JNK2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76125.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 48kDa; JNK2
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JNK2 antibody [EP1595Y] (ab76125)](http://www.abcam.cn/ps/products/76/ab76125/Images/ab76125ihc.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JNK2 antibody [EP1595Y] (ab76125)ab76125 at 1/100 dilution staining JNK2 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue. -
![Western blot - Anti-JNK2 antibody [EP1595Y] (ab76125)](http://www.abcam.cn/ps/products/76/ab76125/Images/ab76125wb.gif)
Western blot - Anti-JNK2 antibody [EP1595Y] (ab76125)Anti-JNK2 antibody [EP1595Y] (ab76125) at 1/50000 dilution + HeLa cell lysate at 10 µg
Secondary
goat anti-rabbit-HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 48 kDa
Observed band size: 54 kDa (why is the actual band size different from the predicted?)
Additional bands at: 46 kDa (possible isoform) -
![Flow Cytometry - Anti-JNK2 antibody [EP1595Y] (ab76125)](http://www.abcam.cn/ps/products/76/ab76125/Images/JNK2-Primary-antibodies-ab76125-2.jpg)
Flow Cytometry - Anti-JNK2 antibody [EP1595Y] (ab76125)Overlay histogram showing HeLa cells stained with ab76125 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76125, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
文献
This product has been referenced in:
- Kitanaka T et al. JNK activation is essential for activation of MEK/ERK signaling in IL-1ß-induced COX-2 expression in synovial fibroblasts. Sci Rep 7:39914 (2017). RT-PCR ; Cat . Read more (PubMed: 28054591) »
- Wang B et al. IL-34 Upregulated Th17 Production through Increased IL-6 Expression by Rheumatoid Fibroblast-Like Synoviocytes. Mediators Inflamm 2017:1567120 (2017). WB ; Human . Read more (PubMed: 28659662) »
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