RabMAb

Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223)抗体[EP1597Y] (ab76572)

概述

  • 产品名称Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223)抗体[EP1597Y]
    参阅全部 JNK1+JNK2+JNK3 一抗
  • 描述
    兔单克隆抗体[EP1597Y] to JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223)
  • 特异性Detects JNK1 (pY185), JNK2 (pY185) and JNK3 (pY223).
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phospho-peptide corresponding to residues surrounding tyrosine 223 of Human JNK3.

  • 阳性对照
    • Lysate from UV-treated Hek293 cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

应用

Our Abpromise guarantee covers the use of ab76572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/20000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/20.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH.
      JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
    • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • 结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • 翻译后修饰Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C Jun kinase 2 antibody
      • c Jun N terminal kinase 1 antibody
      • c Jun N terminal kinase 2 antibody
      • c Jun N terminal kinase 3 antibody
      • c-Jun N-terminal kinase 1 antibody
      • JNK 46 antibody
      • JNK 55 antibody
      • JNK antibody
      • JNK-46 antibody
      • JNK1 antibody
      • JNK1A2 antibody
      • JNK2 antibody
      • JNK21B1/2 antibody
      • JNK2A antibody
      • JNK2ALPHA antibody
      • JNK2B antibody
      • JNK2BETA antibody
      • JNK3 alpha protein kinase antibody
      • JNK3 antibody
      • JNK3A antibody
      • Jun kinase antibody
      • JUN N terminal kinase antibody
      • MAP kinase 10 antibody
      • MAP kinase 8 antibody
      • MAP kinase 9 antibody
      • MAP kinase p49 3F12 antibody
      • MAPK 10 antibody
      • MAPK 8 antibody
      • MAPK 9 antibody
      • MAPK10 antibody
      • mapk8 antibody
      • MAPK9 antibody
      • Mitogen activated protein kinase 10 antibody
      • Mitogen activated protein kinase 8 antibody
      • Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody
      • Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody
      • Mitogen activated protein kinase 9 antibody
      • Mitogen-activated protein kinase 8 antibody
      • MK08_HUMAN antibody
      • p493F12 antibody
      • p54a antibody
      • p54aSAPK antibody
      • p54bSAPK antibody
      • PRKM10 antibody
      • PRKM8 antibody
      • PRKM9 antibody
      • SAPK antibody
      • SAPK(beta) antibody
      • SAPK1 antibody
      • SAPK1a antibody
      • SAPK1b antibody
      • SAPK1c antibody
      • Stress activated protein kinase 1 antibody
      • Stress activated protein kinase 1a antibody
      • Stress activated protein kinase 1b antibody
      • Stress activated protein kinase 1c antibody
      • Stress activated protein kinase beta antibody
      • Stress activated protein kinase JNK1 antibody
      • Stress activated protein kinase JNK2 antibody
      • Stress activated protein kinase JNK3 antibody
      • Stress-activated protein kinase 1c antibody
      • Stress-activated protein kinase JNK1 antibody
      see all

    Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y] 图像

    • All lanes : Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y] (ab76572) at 1/20000 dilution

      Lane 1 : lysates from untreated Hek293 cells
      Lane 2 : lysates from Hek293 cells treated with UV

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 48 kDa
      Observed band size : 48 kDa

    Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y] (ab76572)参考文献

    This product has been referenced in:
    • Yang CY  et al. Dual-specificity phosphatase 14 (DUSP14/MKP6) negatively regulates TCR signaling by inhibiting TAB1 activation. J Immunol 192:1547-57 (2014). Read more (PubMed: 24403530) »
    • Bhattacharyya S  et al. Amelioration of aspirin induced oxidative impairment and apoptotic cell death by a novel antioxidant protein molecule isolated from the herb Phyllanthus niruri. PLoS One 9:e89026 (2014). Mouse . Read more (PubMed: 24586486) »

    See all 6 Publications for this product

    Product Wall

    Application Western blot
    Sample Rat Cell lysate - whole cell (Primary rat hepatocyte)
    Gel Running Conditions Reduced Denaturing (9%)
    Loading amount 20 µg
    Treatment 1mM paracetamol
    Specification Primary rat hepatocyte
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. Philip Probert

    Verified customer

    提交于 Jun 03 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"