概述

  • 产品名称Anti-JNK1+JNK2+JNK3抗体
    参阅全部 JNK1+JNK2+JNK3 一抗
  • 描述
    兔多克隆抗体to JNK1+JNK2+JNK3
  • 特异性ab59227 detects endogenous levels of total JNK1+JNK2+JNK3 protein.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from human JNK1+JNK2+JNK3 around the phosphorylation site of threonine 183/tyrosine 185 (M-M-TP-P-YP-V-V).

  • 阳性对照
    • 293 cell extracts treated with UV (5mins).

性能

应用

Our Abpromise guarantee covers the use of ab59227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: 1/5000.
    WB: 1/500 - 1/1000. Detects bands of approximately 48 and 53 kDa (predicted molecular weight: 48 and 53 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH.
      JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
    • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • 结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • 翻译后修饰Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C Jun kinase 2 antibody
      • c Jun N terminal kinase 1 antibody
      • c Jun N terminal kinase 2 antibody
      • c Jun N terminal kinase 3 antibody
      • c-Jun N-terminal kinase 1 antibody
      • JNK 46 antibody
      • JNK 55 antibody
      • JNK antibody
      • JNK-46 antibody
      • JNK1 antibody
      • JNK1A2 antibody
      • JNK2 antibody
      • JNK21B1/2 antibody
      • JNK2A antibody
      • JNK2ALPHA antibody
      • JNK2B antibody
      • JNK2BETA antibody
      • JNK3 alpha protein kinase antibody
      • JNK3 antibody
      • JNK3A antibody
      • Jun kinase antibody
      • JUN N terminal kinase antibody
      • MAP kinase 10 antibody
      • MAP kinase 8 antibody
      • MAP kinase 9 antibody
      • MAP kinase p49 3F12 antibody
      • MAPK 10 antibody
      • MAPK 8 antibody
      • MAPK 9 antibody
      • MAPK10 antibody
      • mapk8 antibody
      • MAPK9 antibody
      • Mitogen activated protein kinase 10 antibody
      • Mitogen activated protein kinase 8 antibody
      • Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody
      • Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody
      • Mitogen activated protein kinase 9 antibody
      • Mitogen-activated protein kinase 8 antibody
      • MK08_HUMAN antibody
      • p493F12 antibody
      • p54a antibody
      • p54aSAPK antibody
      • p54bSAPK antibody
      • PRKM10 antibody
      • PRKM8 antibody
      • PRKM9 antibody
      • SAPK antibody
      • SAPK(beta) antibody
      • SAPK1 antibody
      • SAPK1a antibody
      • SAPK1b antibody
      • SAPK1c antibody
      • Stress activated protein kinase 1 antibody
      • Stress activated protein kinase 1a antibody
      • Stress activated protein kinase 1b antibody
      • Stress activated protein kinase 1c antibody
      • Stress activated protein kinase beta antibody
      • Stress activated protein kinase JNK1 antibody
      • Stress activated protein kinase JNK2 antibody
      • Stress activated protein kinase JNK3 antibody
      • Stress-activated protein kinase 1c antibody
      • Stress-activated protein kinase JNK1 antibody
      see all

    Anti-JNK1+JNK2+JNK3 antibody 图像

    • All lanes : Anti-JNK1+JNK2+JNK3 antibody (ab59227) at 1/500 dilution

      Lane 1 : 293 cell extract treated with UV (5mins)
      Lane 2 : 293 cell extract treated with UV (5mins) with immunising peptide


      Predicted band size : 48 kDa
      Observed band size : 48,53 kDa (why is the actual band size different from the predicted?)

    Anti-JNK1+JNK2+JNK3 antibody (ab59227)参考文献

    This product has been referenced in:
    • Kang K  et al. Carnosic acid slows photoreceptor degeneration in the Pde6b(rd10) mouse model of retinitis pigmentosa. Sci Rep 6:22632 (2016). WB . Read more (PubMed: 26961159) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (retina)
    Gel Running Conditions Non-reduced Non-Denaturing (Native)
    Loading amount 80 µg
    Treatment 5% Milk for 1h RT
    Specification retina
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

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    Verified customer

    提交于 Apr 15 2015

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
    Sample Mouse Tissue sections (retina)
    Specification retina
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Feb 09 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"