Anti-JNK1+JNK2抗体[279Q38] (ab37228)

概述

  • 产品名称Anti-JNK1+JNK2抗体[279Q38]
    参阅全部 JNK1+JNK2 一抗
  • 描述
    小鼠单克隆抗体[279Q38] to JNK1+JNK2
  • 特异性ab37228 binds to both JNK1 and JNK2 proteins of molecular weight 46 and 54 kDa.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment (Human) from JNKalpha1 expressed in E.coli.

  • 阳性对照
    • Human Jurkat cells, mouse L929 cells and rat L6 cells.

性能

应用

Our Abpromise guarantee covers the use of ab37228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml. Predicted molecular weight:- JNK1: 45 Kda; JNK2: 54 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
    • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • 结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • 翻译后修饰Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
    • Information by UniProt
    • 数据库链接
    • 别名
      • c jun N terminal kinase 2 antibody
      • c-Jun N-terminal kinase 1 antibody
      • cJun N terminal kinase 1 antibody
      • JNK 1 antibody
      • JNK 2 antibody
      • JNK-46 antibody
      • JNK2ALPHA antibody
      • JNK2BETA antibody
      • MAP kinase 8 antibody
      • MAP kinase 9 antibody
      • MAPK 8 antibody
      • mapk8 antibody
      • MAPK9 antibody
      • Mitogen activated protein kinase 8 antibody
      • Mitogen activated protein kinase 9 antibody
      • Mitogen-activated protein kinase 8 antibody
      • MK08_HUMAN antibody
      • PRKM 8 antibody
      • PRKM 9 antibody
      • Stress-activated protein kinase 1 antibody
      • Stress-activated protein kinase JNK1 antibody
      see all

    Anti-JNK1+JNK2 antibody [279Q38] 图像

    • Anti-JNK1+JNK2 antibody [279Q38] (ab37228) at 1 µg/ml + Cell extract of human Jurkat cells (10-30µg/lane total protein)

      Secondary
      Goat F(ab’)2 antimouse IgG, alkaline phosphatase conjugated antibody, at 1/2000 dilution

      Predicted band size : JNK1: 45 ; JNK2: 54 kDa
      Observed band size : 46,54 kDa (why is the actual band size different from the predicted?)

    Anti-JNK1+JNK2 antibody [279Q38] (ab37228)参考文献

    ab37228 has not yet been referenced specifically in any publications.

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