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RabMAb

Anti-JNK1抗体[EPR140(2)] (ab110724)

概述

  • 产品名称
    Anti-JNK1抗体[EPR140(2)]
    参阅全部 JNK1 一抗
  • 描述
    兔单克隆抗体[EPR140(2)] to JNK1
  • 经测试应用
    适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human JNK1.

  • 阳性对照
    • HeLa, 293T, K562, and MCF7 cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab110724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能
      Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
    • 序列相似性
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • 结构域
      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • 翻译后修饰
      Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AI849689 antibody
      • c Jun N terminal kinase 1 antibody
      • C-JUN kinase 1 antibody
      • c-Jun N-terminal kinase 1 antibody
      • EC 2.7.11.24 antibody
      • JNK 1 antibody
      • JNK antibody
      • JNK-46 antibody
      • JNK1A2 antibody
      • JNK21B1/2 antibody
      • MAP kinase 8 antibody
      • MAPK 8 antibody
      • mapk8 antibody
      • Mitogen activated protein kinase 8 antibody
      • Mitogen-activated protein kinase 8 antibody
      • MK08_HUMAN antibody
      • p54 gamma antibody
      • Prkm8 antibody
      • Protein kinase JNK1 antibody
      • Protein kinase, mitogen-activated, 8 antibody
      • SAPK 1 antibody
      • SAPK gamma antibody
      • SAPK1 antibody
      • Stress-activated protein kinase 1 antibody
      • Stress-activated protein kinase JNK1 antibody
      see all

    Anti-JNK1 antibody [EPR140(2)] 图像



    • Predicted band size : 48 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab110724 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

      ab110724 was shown to specifically react with JNK1 when JNK1 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab110724 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 48 kDa


    • Predicted band size : 48 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green).

      Green - target observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

      This western blot image is a comparison between ab110724 and a competitor's top cited mouse monoclonal antibody.

       

    实验方案

    Anti-JNK1 antibody [EPR140(2)] (ab110724)参考文献

    This product has been referenced in:
    • Kitanaka T  et al. JNK activation is essential for activation of MEK/ERK signaling in IL-1ß-induced COX-2 expression in synovial fibroblasts. Sci Rep 7:39914 (2017). Real Time PCR ; Cat . Read more (PubMed: 28054591) »
    • Zhai W  et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain 9:66 (2016). WB ; Rat . Read more (PubMed: 27301321) »

    See all 10 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Cat Cell lysate - whole cell (synovial)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    10 µg
    Treatment
    siRNA for scramble control, JNK1 and JNK2
    Specification
    synovial
    Blocking step
    (agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    令 中野

    Verified customer

    提交于 Jun 30 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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