概述

  • 产品名称JNK Activity Screening试剂盒
  • 测试
    40 x 1 assay
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型Enzyme activity
  • 产品概述

    Abcam's JNK Activity Screening Kit utilizes an N-terminal c-Jun (1-79) fusion protein bound to glutathion sepharose beads to selectively “pull down” JNK from cell lysates. After washing to remove non-specifically bound proteins, the kinase reaction is then carried out in the presence of cold ATP. c-Jun phosphorylation is then measured by Western blot analysis using a phospho-c-Jun specific antibody.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 功能Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt
  • 别名
    • c Jun N terminal kinase 1
    • c-Jun N-terminal kinase 1
    • JNK
    • JNK 1
    • JNK-46
    • JNK1
    • JNK1A2
    • JNK21B1/2
    • JNK46
    • JUN N terminal kinase
    • MAP kinase 8
    • MAPK 8
    • mapk8
    • Mitogen activated protein kinase 8 isoform JNK1 alpha1
    • Mitogen activated protein kinase 8 isoform JNK1 beta2
    • Mitogen-activated protein kinase 8
    • MK08_HUMAN
    • PRKM 8
    • PRKM8
    • Protein kinase JNK1
    • SAPK1
    • SAPK1c
    • Stress activated protein kinase 1c
    • Stress-activated protein kinase 1
    • Stress-activated protein kinase JNK1
    see all

应用

Our Abpromise guarantee covers the use of ab65784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

实验方案

JNK Activity Screening Kit (ab65784)参考文献

ab65784 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab65784.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"