概述

  • 产品名称Anti-JMY抗体
    参阅全部 JMY 一抗
  • 描述
    兔多克隆抗体to JMY
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Bat
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human JMY (NP_689618.3).

  • 阳性对照
    • Colon carcinoma tissue Prostate carcinoma tissue
  • 常规说明Shelf Life: 1 year from date of receipt

性能

应用

Our Abpromise guarantee covers the use of ab125190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Antigen retrieval is recommended using citrate buffer as this will enhance staining.

靶标

  • 功能Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.
  • 序列相似性Belongs to the JMY family.
    Contains 1 WH2 domain.
  • 翻译后修饰Ubiquitinated by MDM2, leading to its subsequent degradation by the proteasome. In case of DNA damage, the interaction with MDM2 is altered, preventing degradation and allowing interaction with p300/EP300 and its function in p53/TP53 stress response.
  • 细胞定位Nucleus. Cytoplasm > cytoskeleton. Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility (By similarity). In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ37870 antibody
    • Jmy antibody
    • JMY protein antibody
    • JMY_HUMAN antibody
    • junction mediating and regulatory protein antibody
    • Junction mediating and regulatory protein p53 cofactor antibody
    • Junction-mediating and -regulatory protein antibody
    • MGC163496 antibody
    • OTTHUMP00000161976 antibody
    • WAS protein homology region 2 domain containing 1-like 3 antibody
    • WHDC1L3 antibody
    see all

Anti-JMY antibody 图像

  • ab125190, at 1/250, staining JMY in formalin fixed, paraffin embedded Human colon carcinoma tissue by Immunohistochemistry. Detected by DAB staining.

Anti-JMY antibody (ab125190)参考文献

ab125190 has not yet been referenced specifically in any publications.

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