Anti-JMJD6抗体(ab50720)

概述

  • 产品名称Anti-JMJD6抗体
    参阅全部 JMJD6 一抗
  • 描述
    兔多克隆抗体to JMJD6
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: VLADSVDLQE STGIASDSSS DSSSSSSSSS SDSDSECESG SEGDGTVHRR, corresponding to C terminal amino acids 325-374 of Human JMJD6

  • 阳性对照
    • Fetal thymus lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab50720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 46 kDa.Can be blocked with JMJD6 peptide (ab110802). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.
  • 组织特异性Highly expressed in the heart, skeletal muscle and kidney. Expressed at moderate or low level in brain, placenta, lung, liver, pancreas, spleen, thymus, prostate, testis and ovary. Up-regulated in many patients with chronic pancreatitis. Expressed in nursing thymic epithelial cells.
  • 序列相似性Belongs to the JMJD6 family.
    Contains 1 JmjC domain.
  • 结构域The nuclear localization signal motifs are necessary and sufficient to target it into the nucleus.
  • 细胞定位Nucleus > nucleoplasm. Nucleus > nucleolus. Mainly found throughout the nucleoplasm outside of regions containing heterochromatic DNA, with some localization in nucleolus. During mitosis, excluded from the nucleus and reappears in the telophase of the cell cycle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptotic cell clearance receptor antibody
    • Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 antibody
    • Histone arginine demethylase JMJD6 antibody
    • JmjC domain-containing protein 6 antibody
    • JMJD 6 antibody
    • JMJD6 antibody
    • JMJD6_HUMAN antibody
    • Jumonji domain containing 6 antibody
    • Jumonji domain-containing protein 6 antibody
    • KIAA0585 antibody
    • Lysyl-hydroxylase JMJD6 antibody
    • Peptide-lysine 5-dioxygenase JMJD6 antibody
    • Phosphatidylserine receptor antibody
    • Protein PTDSR antibody
    • PSR antibody
    • PTDSR 1 antibody
    • PTDSR antibody
    • PTDSR1 antibody
    see all

Anti-JMJD6 antibody 图像

  • Anti-JMJD6 antibody (ab50720) at 1.25 µg/ml + Fetal thymus lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa

Anti-JMJD6 antibody (ab50720)参考文献

ab50720 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Human Cell (293T)
Specification 293T
Permeabilization Yes - Triton
Fixative Formaldehyde
Username

Herr Dr. Alexander Wolf

Verified customer

提交于 Jul 30 2014

Thank you for contacting Abcam.

I am sorry this product has not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a blocking peptide (ab110802) to you with the order number 1021613. Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (N/A)
Loading amount 0.01 µg
Specification N/A
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"