Anti-JMJD5抗体- ChIP Grade (ab83011)

概述

  • 产品名称Anti-JMJD5抗体- ChIP Grade
    参阅全部 JMJD5 一抗
  • 描述
    兔多克隆抗体to JMJD5 - ChIP Grade
  • 经测试应用适用于: ChIP, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human JMJD5 aa 288-337 (internal sequence).
    Sequence:

    DISIPDYCSLGDGEEEEITINAWFGPQGTISPLHQDPQQNFLVQVMGRKY


    Database link: Q8N371

  • 阳性对照
    • Fetal liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab83011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-JMJD5 antibody - ChIP Grade 图像

  • ChIP analysis using ab83011 (0.5µg/mL) binding JMJD5 in human HCT116 colon cancer cells. Without (- peptide) and with (+ peptide) blocking peptide. Protein binding was detected using real-time PCR.

  • Anti-JMJD5 antibody - ChIP Grade (ab83011) at 1 µg/ml + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa

Anti-JMJD5 antibody - ChIP Grade (ab83011)参考文献

ab83011 has not yet been referenced specifically in any publications.

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