Anti-JMJD5抗体(ab28883)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to JMJD5
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human JMJD5. Synthetic peptide derived from residues 350 - 399 of human JMJD5.
    Database link: Q8N371

  • 阳性对照
    • Fetal liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab28883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-JMJD5 antibody (ab28883) at 0.5 µg/ml + Human Fetal Liver Lysate

    Secondary
    HRP conjugated anti-Rabbit IgG (suggested dilution 1:50,000 - 100,000)
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab28883 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Caenorhabditis elegans Tissue lysate - nuclear (All body)
Specification
All body
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Pier Giorgio Amendola

Verified customer

提交于 Jun 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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