概述

  • 产品名称
  • 描述
    兔多克隆抗体to JMJD5
  • 特异性
    ab106391 is specific for JMJD5. At least two isoforms of JMJD5 are known to exist; this antibody should detect both.
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human JMJD5 (C terminal). An 18 amino acid peptide from near the carboxy terminus of Human JMJD5.
    Database link: Q8N371

  • 阳性对照
    • WB: Human liver tissue lysate IHC-P: Human liver tissue

性能

应用

Our Abpromise guarantee covers the use of ab106391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 47 kDa.
IHC-P Use a concentration of 2.5 µg/ml.

靶标

  • 功能
    Histone demethylase required for G2/M phase cell cycle progression. Specifically demethylates dimethylated 'Lys-36' (H3K36me2) of histone H3, an epigenetic repressive mark, thereby acting as a transcription activator. Regulates expression of CCNA1 (cyclin-A1), leading to regulate cancer cell proliferation.
  • 组织特异性
    Weakly expressed in most cells. Highly expressed in breast cancer cells.
  • 序列相似性
    Contains 1 JmjC domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ13798 antibody
    • JmjC domain-containing protein 5 antibody
    • JMJD5 antibody
    • JMJD5 protein antibody
    • jumonji domain containing 5 antibody
    • Jumonji domain-containing protein 5 antibody
    • KDM8_HUMAN antibody
    • Lysine (K) specific demethylase 8 antibody
    • Lysine-specific demethylase 8 antibody
    see all

图片

  • Lane 1 : Anti-JMJD5 antibody (ab106391) at 1 µg/ml
    Lane 2 : Anti-JMJD5 antibody (ab106391) at 2 µg/ml

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 47 kDa
  • ab106391, at 2.5 µg/ml, staining JMJD5 in paraffin embedded Rat liver tissue by Immunohistochemistry.
  • Immunofluorescence of JMJD5 in Rat Liver cells using ab106391 at 20 ug/ml.

文献

This product has been referenced in:
  • Wu BH  et al. Epigenetic silencing of JMJD5 promotes the proliferation of hepatocellular carcinoma cells by down-regulating the transcription of CDKN1A 686. Oncotarget 7:6847-63 (2016). Read more (PubMed: 26760772) »

See 1 Publication for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab106391.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册