The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 µg/ml.
Use a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
功能Probable histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May be involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes.
序列相似性Belongs to the JHDM2 histone demethylase family. Contains 1 JmjC domain.
结构域Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
Probable JmjC domain-containing histone demethylation protein 2C antibody
Thryoid receptor interacting protein antibody
Thyroid receptor-interacting protein 8 antibody
TR-interacting protein 8 antibody
Anti-JMJD1C antibody 图像
Flow Cytometry - Anti-JMJD1C antibody (ab56538)
Overlay histogram showing HeLa cells stained with ab56538 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56538, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
IHC-P - JMJD1C antibody (ab56538)
JMJD1C antibody (ab56538) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
Western blot - Anti-JMJD1C antibody (ab56538)
Western blot against tagged recombinant protein immunogen using ab56538 JMJD1C antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
Anti-JMJD1C antibody (ab56538)参考文献
has not yet been referenced specifically in any publications.