Anti-JMJD1C抗体(ab56538)

概述

  • 产品名称Anti-JMJD1C抗体
    参阅全部 JMJD1C 一抗
  • 描述
    小鼠单克隆抗体to JMJD1C
  • 经测试应用适用于: IHC-P, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: IVMNDQVLEP QNVDPSMVQM TFLDDVVHSL LKGENIGITS RRRSRANQNV NAVHSHYTRA QANSPRPAMN SQAAVPKQNT HQQQQQRSIR PNKRKGSD, corresponding to amino acids 2-100 of Human JMJD1C

性能

应用

Our Abpromise guarantee covers the use of ab56538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Probable histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May be involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes.
  • 序列相似性Belongs to the JHDM2 histone demethylase family.
    Contains 1 JmjC domain.
  • 结构域Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • JHD2C_HUMAN antibody
    • Jmjd1c antibody
    • Jumonji domain containing 1C antibody
    • Jumonji domain-containing protein 1C antibody
    • Probable JmjC domain-containing histone demethylation protein 2C antibody
    • Thryoid receptor interacting protein antibody
    • Thyroid receptor-interacting protein 8 antibody
    • TR-interacting protein 8 antibody
    • TRIP-8 antibody
    • TRIP8 antibody
    see all

Anti-JMJD1C antibody 图像

  • Overlay histogram showing HeLa cells stained with ab56538 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56538, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • JMJD1C antibody (ab56538) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
  • Western blot against tagged recombinant protein immunogen using ab56538 JMJD1C antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

Anti-JMJD1C antibody (ab56538)参考文献

ab56538 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - nuclear (human cell lines)
Loading amount 15 µg
Specification human cell lines
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Aug 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa (cervical cancer cell line))
Loading amount 10 µg
Specification HeLa (cervical cancer cell line)
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"