Anti-JIP3抗体(ab4545)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to JIP3
  • 特异性
    This antibody is expected to recognise both human isoforms of this protein.
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Cow, Human
  • 免疫原

    Synthetic peptide:

    MEIQMDEGGGVV

    , corresponding to N terminal amino acids 2-13 of Human JIP3.

  • 常规说明
    GenBank Accession Number – NP_055948; NP_203750. LocusLink ID - 23162 (human); 30957 (mouse). Gene Ontology terms - vesicle-mediated transport; kinesin complex; cytoplasm; kinesin binding; MAP-kinase scaffold activity; protein kinase binding; regulation of JNK cascade.

性能

应用

Our Abpromise guarantee covers the use of ab4545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interations with the JNK-signaling components and motor proteins.
  • 序列相似性
    Belongs to the JIP scaffold family.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-jun-amino-terminal kinase-interacting protein 3 antibody
    • FLJ00027 antibody
    • Homolog of Drosophila Sunday driver 2 antibody
    • JIP-3 antibody
    • JIP3 antibody
    • JIP3_HUMAN antibody
    • JNK / Stress Activated Protein Kinase Associated Protein 1 antibody
    • JNK Interacting Protein 3 antibody
    • JNK MAP kinase scaffold protein 3 antibody
    • JNK-interacting protein 3 antibody
    • JNK/SAPK associated protein 1 antibody
    • JSAP1 antibody
    • KIAA1066 antibody
    • MAPK8IP3 antibody
    • Mitogen Activated Protein kinase 8 Interacting Protein 3 antibody
    • Mitogen-activated protein kinase 8-interacting protein 3 antibody
    • Syd antibody
    • SYD2 antibody
    see all

图片

  • ab4545 (4µg/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Shows axonal staining in various regions

文献

ab4545 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
60 µg
Specification
heart
Gel Running Conditions
Non-reduced Denaturing (7.5)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

提交于 Feb 15 2012

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Thank you for your enquiry. Ab4545 is a Fast-Track antibody; Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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