概述

  • 产品名称Anti-JDP2抗体
    参阅全部 JDP2 一抗
  • 描述
    兔多克隆抗体to JDP2
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat不与反应: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human JDP2.

    (Peptide available as ab41625.)

  • 阳性对照
    • ab40916 gave a positive signal in the following whole cell lysates: HeLa, HeLa Nuclear, Jurkat Nuclear, HepG2 Nuclear, Du145 and LnCAP Nuclear.

性能

应用

Our Abpromise guarantee covers the use of ab40916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Component of the AP-1 transcription factor that represses transactivation mediated by the Jun family of proteins. Involved in a variety of transcriptional responses associated with AP-1 such as UV-induced apoptosis, cell differentiation, tumorigenesis and antitumogeneris. Can also function as a repressor by recruiting histone deacetylase 3/HDAC3 to the promoter region of JUN. May control transcription via direct regulation of the modification of histones and the assembly of chromatin.
  • 序列相似性Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • 翻译后修饰Phosphorylation of Thr-148 by MAPK8 in response to different stress conditions such as, UV irradiation, oxidatives stress and anisomycin treatments.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Jdp2 antibody
    • JDP2_HUMAN antibody
    • Jun dimerization protein 2 antibody
    • JUNDM2 antibody
    • Progesterone receptor co-activator antibody
    see all

Anti-JDP2 antibody 图像

  • All lanes : Anti-JDP2 antibody (ab40916) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : Jurkat nuclear extract lysate (ab14844)
    Lane 5 : HepG2 nuclear extract lysate (ab14660)
    Lane 6 : Du145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 7 : LNCaP (Human prostate adenocarcinoma cell line) Nuclear lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 19 kDa
  • ICC/IF image of ab40916 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40916, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab40916 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40916, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-JDP2 antibody (ab40916)参考文献

This product has been referenced in:
  • Tanigawa S  et al. Jun dimerization protein 2 is a critical component of the Nrf2/MafK complex regulating the response to ROS homeostasis. Cell Death Dis 4:e921 (2013). WB ; Human . Read more (PubMed: 24232097) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (CD4+ T cells)
Loading amount 1e+006 cells
Specification CD4+ T cells
Gel Running Conditions Non-reduced Denaturing (4 - 12 % gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Jun 26 2008

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