JC-10 Mitochondrial膜Potential Assay试剂盒(Flow Cytometry) (ab112133)

概述

  • 产品名称JC-10 Mitochondrial膜Potential Assay试剂盒(Flow Cytometry)
    参阅全部 Mitochondrial Transmembrane Potential 试剂盒
  • 测试
    1 x 100 test
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Direct
  • 产品概述

    JC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab112133) enables researchers to run JC-10 assay using flow cytometry, and it provides the most robust assay method for monitoring mitochondria membrane potential changes. This assay is based on the detection of the mitochondrial membrane potential changes in cells by the cationic, lipophilic JC-10 dye. In normal cells, JC-10 concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. However, in apoptotic and necrotic cells, JC-10 diffuses out of mitochondria. It changes to monomeric form and stains cells in green fluorescence.


    Although JC-1 is widely used in many labs, its poor water solubility causes great inconvenience. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 is developed to be a superior alternative to JC-1 when high dye concentration is desired. Compared to JC-1, JC-10 has much better water solubility. JC-10 is capable of selectively entering into mitochondria, and reversibly changes its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (ie., emission of JC-10 monomeric form) to 570 nm (ie., emission of J-aggregate form). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized.


    In normal cells, JC-10 concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. However, in apoptotic and necrotic cells, JC-10 exists in monomeric form and stains cells green. The green emission can be analyzed in fluorescence channel 1 (FL1) and greenish orange emission in channel 2 (FL2). Both colors can be detected using the filters commonly mounted in all flow cytometers. Besides its use in flow cytometry, it can also be used in fluorescence imaging and fluorescence microplate platform.

    Visit our FAQs page for tips and troubleshooting.

  • 说明

    If you would like to use JC-10 on a microplate reader, we recommend JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) (ab112134).

  • 经测试应用适用于: Flow Cytmore details

性能

  • 相关性Mitochondrial Transmembrane Potential is an important parameter of mitochondrial function used as an indicator of cell death. The collapse of the mitochondrial transmembrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
  • 别名
    • mitochondrial membrane potential

相关产品

应用

Our Abpromise guarantee covers the use of ab112133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

JC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) 图像

  • ab112133 was used to measure the effect of FCCP induced mitochondria membrane potential change in Jurkat cells by Flow Cytometry. Jurkat cells were dye loaded with JC-10 dye-loading solution along with DMSO (Top) or 5 µM FCCP (Low) for 10 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-10 were measured with a flow cytometer using FL1 and FL2 channels. Uncompensated data (left column) were compared with compensated data (right column).

实验方案

JC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab112133)参考文献

This product has been referenced in:
  • Khurana S  et al. Antiapoptotic actions of methyl gallate on neonatal rat cardiac myocytes exposed to H2O2. Oxid Med Cell Longev 2014:657512 (2014). Flow Cyt ; Rat . Read more (PubMed: 24672637) »
  • Liu Z  et al. Potent Half-Sandwich Iridium(III) Anticancer Complexes Containing C(?)N-Chelated and Pyridine Ligands. Organometallics 33:5324-5333 (2014). Read more (PubMed: 25328266) »

See all 2 Publications for this product

Product Wall





This kit is for live cells only.



Based on the data, it looks like the way your customer treated the cells probably is the reason for the problem. It most likely when detaching the cells (trypsinized?), the mitochondria membrane potential changes. If you have to use flow, might try EDT...

Read More



The concentration of CCCP or FCCP required as a positive control will vary based on cell line. Perhaps it would be useful to try 50uM FCCP as this has worked for you in the past.

Thank you for providing that extra information.

I have talked to the lab again, to see if they have any other thoughts on this and they think it is probably due to using the different "gating method", attached please find a copy of ...

Read More

Thank you for providing that information.

I have talked to our supplier about your concerns and they have told me that your results are most likely due to the method of preparing the cells. Physically scraped the cells may damage the cell mem...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"