Janus Green cell normalization stain (ab111622)

概述

  • 产品名称Janus Green cell normalization stain
  • 经测试应用适用于: In-Cell ELISAmore details
  • 常规说明Cells fixed to a solid surface (typically in microplate wells) can be stained by adding 1X Janus Green Stain. Incubate cells for 5 minutes at room temperature. Dye should be removed and washed 5 times in ultrapure water or until excess dye is removed. The last wash should be removed and add 0.1 mL 0.5 M HCl per well and incubate for 10 minutes. Shake the plate for 10 seconds, and measure the OD 595 nm using a standard microplate spectrophotometer.

性能

  • 形式Liquid
  • 存放说明Store at +4°C.
  • 存储溶液Constituents: 0.02% Monobasic dihydrogen potassium phosphate, 0.216% Dibasic monohydrogen sodium phosphate, 0.02% Potassium chloride, 0.81% Sodium chloride, Water, 0.3% Janus Green B
  • Concentration information loading...
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab111622 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    In-Cell ELISA Use at an assay dependent dilution. 50 µL/ well fixed cells

    Janus Green cell normalization stain (ab111622)参考文献

    ab111622 has not yet been referenced specifically in any publications.

    Product Wall

    Yes, the OD 595 can be read directly on the plate where the cells were grown and the assay was performed. The HCL will disrupt the cells and the janus green (which stains the nuclei) will be released into the well. No need to spin or move the HCL to a ...

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    Thank you for your enquiry,

    We recommend to measure the assays results at OD595 nm using a standard microplate spectrophotometer. We will be amending the datasheet to make this clearer.

    Whole Cell Staining with Janus Green:
    ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"