The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
组织特异性Highly expressed in brain, moderately expressed in thymus, spleen and lung, and weakly expressed in kidney, liver and peripheral blood lymphocytes. Aslo expressed in adrenal and pituitary glands, as well as testis.
ab65019, at 1/50 - 1/100 dilution, staining JAKMIP2 in human brain by immunohistochemistry using paraffin-embedded tissue, in the absence (left panel) or presence (right panel) of the immunising peptide.
Western blot - JAKMIP2 antibody (ab65019)
All lanes : Anti-JAKMIP2 antibody (ab65019) at 1/500 dilution
Lane 1 : LOVO cell extract Lane 2 : A549 cell extract Lane 3 : A549 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 95 kDa Observed band size : 95 kDa
Anti-JAKMIP2 antibody (ab65019)参考文献
has not yet been referenced specifically in any publications.