Anti-JAK3抗体(ab78116)

概述

  • 产品名称Anti-JAK3抗体
    参阅全部 JAK3 一抗
  • 描述
    兔多克隆抗体to JAK3
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide:

    SDPTPGIPSPRDELC

    , corresponding to amino acids 785 - 799 of Mouse JAK3

  • 阳性对照
    • HeLa cell lysate, Jurkat cell lysate and HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab78116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Predicted molecular weight: 125 kDa.
IP Use at an assay dependent dilution.

靶标

  • 功能Tyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K.
  • 组织特异性In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.
  • 疾病相关Defects in JAK3 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-negative (T(-)B(+)NK(-) SCID) [MIM:600802]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • 结构域Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
  • 翻译后修饰Tyrosine phosphorylated in response to IL-2 and IL-4.
  • 细胞定位Endomembrane system. Wholly intracellular, possibly membrane associated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.7.10.2 antibody
    • JAK 3 antibody
    • JAK L antibody
    • JAK-3 antibody
    • Jak3 antibody
    • JAK3 HUMAN antibody
    • JAK3_HUMAN antibody
    • JAKL antibody
    • Janus kinase 3 (a protein tyrosine kinase, leukocyte) antibody
    • Janus kinase 3 antibody
    • Janus Kinase3 antibody
    • L JAK antibody
    • L-JAK antibody
    • Leukocyte janus kinase antibody
    • LJAK antibody
    • Protein tyrosine kinase leukocyte antibody
    • Tyrosine protein kinase JAK3 antibody
    • Tyrosine-protein kinase JAK3 antibody
    see all

Anti-JAK3 antibody 图像

  • Anti-JAK3 antibody (ab78116) at 1/500 dilution + HeLa cell lysate

    Secondary
    donkey anti-rabbit HRP conjugated IgG at 1/1000 dilution

    Predicted band size : 125 kDa
    Observed band size : 125 kDa
  • All lanes : Anti-JAK3 antibody (ab78116) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate

    Secondary
    donkey anti-rabbit HRP conjugated IgG at 1/1000 dilution

    Predicted band size : 125 kDa
    Observed band size : 125 kDa

Anti-JAK3 antibody (ab78116)参考文献

ab78116 has not yet been referenced specifically in any publications.

Product Wall

I am sorry to hear that the antibody still giving you wrong band size. The antibody should have given ˜120 kDa band however we are unsure about the cause of the problem. In order to proceed further, I am happy to provide the replacement which I agreed ...

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Thank you very much or confirming details.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"