Anti-JAK2 (phospho Y1007 + Y1008)抗体(ab68268)

概述

  • 产品名称Anti-JAK2 (phospho Y1007 + Y1008)抗体
    参阅全部 JAK2 一抗
  • 描述
    兔多克隆抗体to JAK2 (phospho Y1007 + Y1008)
  • 特异性This antibody recognizes JAK2 with dual phosphorylated sites of Tyr1007/1008. It does not crossreact with non-phosphospecific peptide.
  • 经测试应用适用于: ICC/IF, Dot blot, WB, IP, Indirect ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide surrounding to the epitope -EYYK- with dual phosphorylation sites at Tyr1008 and Tyr1009 of JAK2 from human, mouse and rat origins.

  • 阳性对照
    • GH stimulated mouse liver tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab68268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Dot blot
WB
IP
Indirect ELISA
  • 应用说明Dot: Use at an assay dependent dilution.
    IP: Use at a concentration of 2 - 5 µg/ml.
    I-ELISA: Use at a concentration of 0.01 - 0.1 µg/ml.
    WB: Use at a concentration of 0.1 - 1 µg/ml. Detects bands of approximately 125 and 140 kDa (predicted molecular weight: 130 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Non-receptor tyrosine kinase involved in various processes such as cell cycle progression, apoptosis, mitotic recombination, genetic instability and histone modifications. In the cytoplasm, plays a pivotal role in signal transduction via its association with cytokine receptors, which constitutes an initiating step in signaling for many members of the cytokine receptor superfamily including the receptors for growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), granulocyte-macrophage colony-stimulating factor (CSF2), thrombopoietin (THPO) and multiple interleukins. Following stimulation with erythropoietin (EPO) during erythropoiesis, it is autophosphorylated and activated, leading to its association with erythropoietin receptor (EPOR) and tyrosine phosphorylation of residues in the EPOR cytoplasmic domain. Also involved in promoting the localization of EPOR to the plasma membrane. Also acts downstream of some G-protein coupled receptors. Plays a role in the control of body weight (By similarity). Mediates angiotensin-2-induced ARHGEF1 phosphorylation. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    • 组织特异性Expressed in blood, bone marrow and lymph node.
    • 疾病相关Note=Chromosomal aberrations involving JAK2 are found in both chronic and acute forms of eosinophilic, lymphoblastic and myeloid leukemia. Translocation t(8;9)(p22;p24) with PCM1 links the protein kinase domain of JAK2 to the major portion of PCM1. Translocation t(9;12)(p24;p13) with ETV6.
      Defects in JAK2 are a cause of susceptibility to Budd-Chiari syndrome (BCS) [MIM:600880]. It is a syndrome caused by obstruction of hepatic venous outflow involving either the hepatic veins or the terminal segment of the inferior vena cava. Obstructions are generally caused by thrombosis and lead to hepatic congestion and ischemic necrosis. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain and abdominal ascites. Budd-Chiari syndrome is associated with a combination of disease states including primary myeloproliferative syndromes and thrombophilia due to factor V Leiden, protein C deficiency and antithrombin III deficiency. Budd-Chiari syndrome is a rare but typical complication in patients with polycythemia vera.
      Defects in JAK2 are a cause of polycythemia vera (PV) [MIM:263300]. A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly.
      Defects in JAK2 gene may be a cause of essential thrombocythemia (ET) [MIM:187950]. ET is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
      Defects in JAK2 are a cause of myelofibrosis (MYELOF) [MIM:254450]. Myelofibrosis is a disorder characterized by replacement of the bone marrow by fibrous tissue, occurring in association with a myeloproliferative disorder. Clinical manifestations may include anemia, pallor, splenomegaly, hypermetabolic state, petechiae, ecchymosis, bleeding, lymphadenopathy, hepatomegaly, portal hypertension.
      Defects in JAK2 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
    • 结构域Possesses 2 protein kinase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for protein kinase 1.
    • 翻译后修饰Autophosphorylated, leading to regulate its activity. Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813. Autophosphorylation on Tyr-119 in response to EPO down-regulates its kinase activity. Autophosphorylation on Tyr-868, Tyr-966 and Tyr-972 in response to growth hormone (GH) are required for maximal kinase activity.
    • 细胞定位Endomembrane system. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • JAK 2 antibody
      • JAK-2 antibody
      • JAK2 antibody
      • JAK2_HUMAN antibody
      • Janus Activating Kinase 2 antibody
      • Janus kinase 2 (a protein tyrosine kinase) antibody
      • Janus kinase 2 antibody
      • JTK 10 antibody
      • JTK10 antibody
      • kinase Jak2 antibody
      • OTTHUMP00000043260 antibody
      • THCYT3 antibody
      • Tyrosine protein kinase JAK2 antibody
      • Tyrosine-protein kinase JAK2 antibody
      see all

    Anti-JAK2 (phospho Y1007 + Y1008) antibody 图像

    • Anti-JAK2 (phospho Y1007 + Y1008) antibody (ab68268) at 1/500 dilution + GH stimulated mouse liver tissue lysate

      Predicted band size : 130 kDa
      Observed band size : 125 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.
    • 1µg peptide was blot onto NC membrane.
      A: JAK2 (pY1008, pY1009).
      B: JAK2 (Non-phosphospecific).
      C: Non-related Phosphopeptide.
      were blotted at a 1:2000 dilution by:
      1: ab68268.
      2: Rabbit anti-JAK2 (Non-phospho specific).
    • Immunofluorescence analysis of U2-OS cells, staining JAK2 (phospho Y1007 + Y1008) with ab43635.

      Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% serum for 2 hours at 20°C. Samples were incubated with primary antibody (1/100 in diluent) for 2 hours at 37°C. An AlexaFluor®488-conjugated chicken anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody.

      See Abreview

    Anti-JAK2 (phospho Y1007 + Y1008) antibody (ab68268)参考文献

    This product has been referenced in:
    • Mishra V  et al. Sex-specific IL-6-associated signaling activation in ozone-induced lung inflammation. Biol Sex Differ 7:16 (2016). Mouse . Read more (PubMed: 26949510) »
    • Ruiz PA & Jarai G Discoidin domain receptors regulate the migration of primary human lung fibroblasts through collagen matrices. Fibrogenesis Tissue Repair 5:3 (2012). WB ; Human . Read more (PubMed: 22336030) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (murine embryonic fibroblasts)
    Specification murine embryonic fibroblasts
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2% Triton-X-100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
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    提交于 Dec 17 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (U2-OS)
    Specification U2-OS
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2% Triton-X-100
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 16 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"