概述

  • 产品名称Anti-Jagged1抗体[EPR4290]
    参阅全部 Jagged1 一抗
  • 描述
    兔单克隆抗体[EPR4290] to Jagged1
  • 经测试应用适用于: WB, IHC-P, IHC-Frmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Jagged1 aa 1150-1250 (C terminal).

  • 阳性对照
    • HepG2 and NIH:OVCAR-3 cell lysate. Bladder cancer tissue. HeLa cells.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 134 kDa.
IHC-P 1/200 - 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

IHC-Fr Use at an assay dependent concentration. PubMed: 24901044
  • 应用说明Is unsuitable for ICC/IF.
  • 靶标

    • 功能Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
    • 组织特异性Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
    • 疾病相关Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
      Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
    • 序列相似性Contains 1 DSL domain.
      Contains 15 EGF-like domains.
    • 发展阶段Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AGS antibody
      • AHD antibody
      • AWS antibody
      • CD 339 antibody
      • CD339 antibody
      • CD339 antigen antibody
      • Headturner antibody
      • hJ1 antibody
      • Htu antibody
      • Jag 1 antibody
      • Jag1 antibody
      • JAG1_HUMAN antibody
      • Jagged 1 antibody
      • Jagged1 (Alagille syndrome) antibody
      • Jagged1 antibody
      • JAGL1 antibody
      • MGC104644 antibody
      • OTTHUMP00000030278 antibody
      • Protein jagged-1 antibody
      • Ser 1 antibody
      • Ser1 antibody
      • Serrate 1 antibody
      • Slalom antibody
      see all

    Anti-Jagged1 antibody [EPR4290] 图像

    • Immunohistochemical staining of paraffin embedded human kidney using purified ab109536 at a dilution of 1/300. A pre-diluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. 

    • Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/1000 dilution (Purified) + HepG2 at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 134 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution (Unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : NIH:OVCAR-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 134 kDa
    • Immunohistochemical staining of Jagged1 in paraffin-embedded bladder cancer tissue using unpurified ab109536 at a dilution of 1/250.

    Anti-Jagged1 antibody [EPR4290] (ab109536)参考文献

    This product has been referenced in:
    • Zhang X  et al. FADD regulates thymocyte development at the ß-selection checkpoint by modulating Notch signaling. Cell Death Dis 5:e1273 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24901044) »
    • Yang W  et al. Ptpn11 deletion in a novel progenitor causes metachondromatosis by inducing hedgehog signalling. Nature 499:491-5 (2013). Mouse . Read more (PubMed: 23863940) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (4-15%)
    Sample Human Cell lysate - whole cell (non small cell lung cancer)
    Specification non small cell lung cancer
    Treatment siRNA treated
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 Oct 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"