概述

  • 产品名称
    Anti-Jagged1抗体
    参阅全部 Jagged1 一抗
  • 描述
    兔多克隆抗体to Jagged1
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide: N-KASRGNDRNRIVLPFS conjugated to KLH, corresponding to amino acids 110-125 of Human Jagged1

性能

应用

Our Abpromise guarantee covers the use of ab7771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100.
WB 1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 134 kDa). 150 kDa in mouse liver lysate and a 75 kDa band in human brain and kidney lysates.

靶标

  • 功能
    Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
  • 组织特异性
    Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
  • 疾病相关
    Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
    Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
  • 序列相似性
    Contains 1 DSL domain.
    Contains 15 EGF-like domains.
  • 发展阶段
    Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGS antibody
    • AHD antibody
    • AWS antibody
    • CD 339 antibody
    • CD339 antibody
    • CD339 antigen antibody
    • Headturner antibody
    • hJ1 antibody
    • Htu antibody
    • Jag 1 antibody
    • Jag1 antibody
    • JAG1_HUMAN antibody
    • Jagged 1 antibody
    • Jagged1 (Alagille syndrome) antibody
    • Jagged1 antibody
    • JAGL1 antibody
    • MGC104644 antibody
    • OTTHUMP00000030278 antibody
    • Protein jagged-1 antibody
    • Ser 1 antibody
    • Ser1 antibody
    • Serrate 1 antibody
    • Slalom antibody
    see all

图片

  • Formalin-fixed paraffin embedded IHC of normal human cervial tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin

  • Staining of Human corneal epithelial cells with anti-Jagged1 (ab7771, 1/500). The Jagged1 (green staining) is localised to the cytoplasm and cell nucleus and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (1/500, blue staining).

  • Formalin-fixed paraffin embedded IHC of human cervical cancer tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin


  • Predicted band size : 134 kDa

    ab7771 Rabbit polyclonal to Jagged1 (1/500) with secondary Goat anti-rabbit IgG antibody ab6721 (1/5000)
    Exposure time: 1 minute. Expected molecular weight: 134 kDa

    Lanes 1 to 3 and 5: 20µg of cell lysate per lane
    Lane 4: 20 µl of 1x LDS loading buffer

    Lane 1: Human Brain tissue lysate
    Lane 2: Human Kidney tissue Lysate
    Lane 3: Human liver tissue lysate
    Lane 4: 1x LDS loading buffer
    Lane 5: Mouse liver lysate

     

文献

This product has been referenced in:

See all 29 Publications for this product

客户评价及客户问答

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from the Anti-Jagged1 antibody (ab7771) .

I would like to reassure you that this antibody is tested and covered by our 6 mon...

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I have heard back for the supplying lab.

The IHC data we have on the datasheet were contributed by their endusers. Unfortunately, they do not have a specific protocol available and all conditions must be user optimized.

I am sorry to ...

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Thank you for your reply and the image.

In the image I am not quite able to tell where the nuclei or potential plasma membranes are as there is no reference point. Does the image reflect just 1 cell or a line up of cells?
Would you have an...

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Thank you for contacting us.

I am checking with the lab regarding the protocol, but could you please send me in the meantime an image showing the staining you are obtaining?

I look forward to your reply and helping you further.
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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for taking time tocontact us. I am sorry to hear that this antibody is not providing satisfactory results.

Are the samples you are staining the same as you have tried previously?

What dilutions of the antibody have you t...

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Thank you for your reply. For Jagged1, I would recommend ab7771, as it guaranteed to work in IHC-P on mouse tissue. As for the positive control, according to www.proteinatlas.org, Jagged1 is highly expressed in liver and pancreas, there is also stainin...

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Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab7771 in IHC. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support but please bear in mi...

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Thank you for your enquiry. I looked at the SwissProt entry for Jagged1 and found that the epitope raised by this antibody is indeed extracellular. The extracellular domain is from amino acids 34-1067, the transmembrane domain is from 1068 to 1093,...

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I'm sorry to hear you are having a problem with ab7771. This antibody is a FastTrack antibody so we have not fully characterised it yet. As you can see from the image on the datasheet it recognises more than one band (polyclonal antibody) in human bra...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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