Anti-Jagged 2抗体(ab80671)

概述

  • 产品名称Anti-Jagged 2抗体
    参阅全部 Jagged 2 一抗
  • 描述
    兔多克隆抗体to Jagged 2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Pig
  • 免疫原

    Synthetic peptide derived from a region within N terminal amino acids 1-50 (MRAQGRGRLP RRLLLLLALW VQAARPMGYF ELQLSALRNV NGELLSGACC) of Human Jagged 2

  • 阳性对照
    • Jurkat membrane lysate

性能

应用

Our Abpromise guarantee covers the use of ab80671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 133 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性Jagged 2 is a Notch ligand involved in the mediation of Notch signaling. Jagged 2 is expressed in heart, placenta and skeletal muscle and to a lesser extend in pancreas. Very low expression in brain, lung, liver and kidney. The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. Jagged 2 is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for Jagged 2.
  • 细胞定位Cell Membrane
  • 数据库链接
  • 别名
    • D12Ggc2e antibody
    • HJ 2 antibody
    • HJ2 antibody
    • JAG 2 antibody
    • JAG2 antibody
    • Jagged 2 precursor antibody
    • Jagged2 antibody
    • Protein jagged 2 antibody
    • SER2 antibody
    • Syndactylism antibody
    see all

Anti-Jagged 2 antibody 图像

  • Anti-Jagged 2 antibody (ab80671) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 133 kDa
    Observed band size : 133 kDa
    Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.

Anti-Jagged 2 antibody (ab80671)参考文献

ab80671 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Embryos -GD9)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 20 µg
Treatment hydroxyurea
Specification Embryos -GD9
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Nazem El Husseini

Verified customer

提交于 May 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"