概述

  • 产品名称
    Anti-JAB1抗体[EPR1350]
    参阅全部 JAB1 一抗
  • 描述
    兔单克隆抗体[EPR1350] to JAB1
  • 经测试应用
    适用于: WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human JAB1.

  • 阳性对照
    • NIH 3T3, U87-MG, MCF7, and PC12 cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab124720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 功能
      Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex.
    • 序列相似性
      Belongs to the peptidase M67A family. CSN5 subfamily.
      Contains 1 MPN (JAB/Mov34) domain.
    • 结构域
      The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex.
    • 细胞定位
      Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 38 kDa Mov34 homolog antibody
      • COP9 (constitutive photomorphogenic) homolog subunit 5 antibody
      • COP9 constitutive photomorphogenic homolog subunit 5 antibody
      • COP9 signalosome complex subunit 5 antibody
      • COP9 signalosome subunit 5 antibody
      • Cop9 subunit 5 antibody
      • COPS 5 antibody
      • cops5 antibody
      • CSN 5 antibody
      • CSN5 antibody
      • CSN5_HUMAN antibody
      • JAB 1 antibody
      • Jun activation domain binding protein 1 antibody
      • Jun activation domain binding protein antibody
      • Jun activation domain-binding protein 1 antibody
      • MGC3149 antibody
      • MOV 34 antibody
      • MOV34 antibody
      • MOV34 family, 38-KD member antibody
      • SGN 5 antibody
      • SGN5 antibody
      • Signalosome subunit 5 antibody
      see all

    图片

    • All lanes : Anti-JAB1 antibody [EPR1350] (ab124720) at 1/1000 dilution

      Lane 1 : NIH 3T3 cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : PC12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 38 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    This product has been referenced in:
    • Coleman KE  et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017). Read more (PubMed: 28475037) »
    • Wang R  et al. COPS5 protein overexpression increases amyloid plaque burden, decreases spinophilin-immunoreactive puncta, and exacerbates learning and memory deficits in the mouse brain. J Biol Chem 290:9299-309 (2015). Mouse . Read more (PubMed: 25713139) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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