The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
功能Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.
组织特异性Expressed in intestinal crypt and villus epithelial cells.
序列相似性Belongs to the InsP3 receptor family. Contains 5 MIR domains.
结构域The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
翻译后修饰Phosphorylated on tyrosine residues. Phosphorylated by AKT1 on serine and/or threonine residues.
ab113292, at 1/250 dilution, staining ITPR3 in formalin-fixed, paraffin-embedded Human stomach carcinoma by Immunohistochemistry. Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100.
Anti-ITPR3 antibody (ab113292)参考文献
has not yet been referenced specifically in any publications.