Anti-ITM2C抗体(ab101389)

概述

  • 产品名称Anti-ITM2C抗体
    参阅全部 ITM2C 一抗
  • 描述
    兔多克隆抗体to ITM2C
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Pig
  • 免疫原

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 110 and 258 of ITM2C (AAH02424).

  • 阳性对照
    • HeLa, HepG2, MCF7 cell lines.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab101389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10mM Citrate buffer or Tris-EDTA buffer (PH8.0)

靶标

  • 功能Negative regulator of beta amyloid peptide production. May inhibit the processing of APP by blocking its access to alpha-and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation.
  • 组织特异性High levels in the brain, specifically in the cerebral cortex, medulla, amygdala, hippocampus, thalamus, caudate nucleus, cerebellum, olfactory lobe and spinal cord. Very low levels in other organs.
  • 序列相似性Belongs to the ITM2 family.
    Contains 1 BRICHOS domain.
  • 翻译后修饰Type I membrane-bound, as well as soluble, furin has a pre-eminent role in ITM2C proteolytic processing. PCSK7 and PCSK5 may also be involved although to a lesser extent. The soluble form of PCSK7 is incapable of processing ITM2C.
  • 细胞定位Lysosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BRI3 antibody
    • BRICD2C antibody
    • BRICHOS domain containing 2C antibody
    • Cerebral protein 14 antibody
    • CT-BRI3 antibody
    • E25 antibody
    • E25C antibody
    • Integral membrane protein 2C antibody
    • Integral membrane protein 3 antibody
    • Itm2c antibody
    • ITM2C_HUMAN antibody
    • ITM3 antibody
    • NPD018 antibody
    • Transmembrane protein BRI3 antibody
    see all

Anti-ITM2C antibody 图像

  • Anti-ITM2C antibody (ab101389) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 30 kDa
  • Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ab101389 at 1/100 dilution.

Anti-ITM2C antibody (ab101389)参考文献

ab101389 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Other - Dynabeads
Sample Human Cell lysate - whole cell (SH-SY5Y)
Specification SH-SY5Y
Total protein in input 1000 µg
Username

Ms. Filipa Martins

Verified customer

提交于 Jun 20 2014

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (5-20% gradient)
Sample Rat Tissue lysate - whole (Cortex)
Specification Cortex
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Ms. Filipa Martins

Verified customer

提交于 Nov 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"