Anti-ITM2B抗体(ab101522)
Key features and details
- Rabbit polyclonal to ITM2B
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-ITM2B抗体
参阅全部 ITM2B 一抗 -
描述
兔多克隆抗体to ITM2B -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Pig -
免疫原
Recombinant fragment corresponding to Human ITM2B aa 65-234.
Database link: Q9Y287 -
阳性对照
- WB: HepG2, HCT116 whole cell lysate. Mouse brain lysate. Rat brain tissue extract. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab101522于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 30 kDa.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 30 kDa. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Functions as a protease inhibitor. Plays a role in APP processing regulating the physiological production of the beta amyloid peptide. Restricts docking of gamma-secretase to APP and access of alpha- and beta-secretase to their cleavage APP sequence. -
组织特异性
Expressed in brain and in other tissues. -
疾病相关
Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 1 (CAA-ITM2B1) [MIM:176500]. A disorder characterized by amyloid deposition in the walls of cerebral blood vessels and neurodegeneration in the central nervous system. Cerebral amyloid angiopathy, non-neuritic and perivascular plaques and neurofibrillary tangles are the predominant pathological lesions. Clinical features include progressive mental deterioration, spasticity and muscular rigidity.
Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 2 (CAA-ITM2B2) [MIM:117300]; also known as heredopathia ophthalmo-oto-encephalica. A disorder characterized by amyloid deposition in the walls of the blood vessels of the cerebrum, choroid plexus, cerebellum, spinal cord and retina. Plaques and neurofibrillary tangles are observed in the hippocampus. Clinical features include progressive ataxia, dementia, cataracts and deafness. -
序列相似性
Belongs to the ITM2 family.
Contains 1 BRICHOS domain. -
翻译后修饰
The C-terminal part of the ectodomain is processed by furin and related proteases producing a secreted peptide of 4 to 5 kDa. For the ABRI and ADAN variants the C-terminal secreted peptide is larger and may produce amyloid fibrils responsible for neuronal dysfunction and dementia. The remaining part of the ectodomain containing the BRICHOS domain is cleaved by ADAM10 and is secreted as a peptide of 25 kDa. The membrane-bound N-terminal fragment (NTF) of 22 kDa is further proteolytically processed by SPPL2A and SPPL2B through regulated intramembrane proteolysis producing a secreted peptide (BRI2C) and an intracellular domain (ICD) released in the cytosol. -
细胞定位
Golgi apparatus membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 510575 Cow
- Entrez Gene: 9445 Human
- Entrez Gene: 16432 Mouse
- Entrez Gene: 595120 Pig
- Entrez Gene: 290364 Rat
- Omim: 603904 Human
- SwissProt: Q3T0P7 Cow
- SwissProt: Q9Y287 Human
see all -
别名
- ABRI antibody
- ABri/ADan amyloid peptide antibody
- BRI 2 antibody
see all
图片
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All lanes : Anti-ITM2B antibody (ab101522) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 30 kDa -
HeLa cells stained for ITM2B (green) using ab101522 at 1/200 dilution in ICC/IF.
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Anti-ITM2B antibody (ab101522) at 1/1000 dilution + Rat brain tissue extract at 50 µg
Predicted band size: 30 kDa12% SDS-PAGE
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Anti-ITM2B antibody (ab101522) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 30 kDa12 % SDS-PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab101522 尚未被引用在任何文献中。