概述

  • 产品名称
    Anti-ITK抗体[Y402]
    参阅全部 ITK 一抗
  • 描述
    兔单克隆抗体[Y402] to ITK
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human ITK (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • Jurkat cell lysate.
  • 常规说明

     Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.21% BSA
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    Y402
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab32507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

For unpurified use at 1/1000.

IP 1/20.

For unpurified use at 1/50.

  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF or IHC.
  • 靶标

    • 功能
      Plays a role in T-cell proliferation and differentiation.
    • 组织特异性
      T-cell lines and natural killer cell lines.
    • 疾病相关
      Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
    • 序列相似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
      Contains 1 Btk-type zinc finger.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • 细胞定位
      Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EMT antibody
      • Homolog of mouse T cell itk/tsk antibody
      • IL 2 inducible T cell kinase antibody
      • IL2 inducible T cell kinase antibody
      • Interleukin 2 inducible T cell kinase antibody
      • Itk antibody
      • ITK_HUMAN antibody
      • Kinase EMT antibody
      • LPFS1 antibody
      • LYK antibody
      • MGC126257 antibody
      • MGC126258 antibody
      • PSCTK 2 antibody
      • PSCTK2 antibody
      • T cell specific kinase antibody
      • T-cell-specific kinase antibody
      • TSK antibody
      • Tyrosine protein kinase antibody
      • Tyrosine protein kinase ITK/TSK antibody
      • Tyrosine protein kinase Lyk antibody
      • Tyrosine-protein kinase ITK/TSK antibody
      • Tyrosine-protein kinase Lyk antibody
      see all

    图片

    • ab32507 (purified) at 1:20 dilution (1.0μg) immunoprecipitating ITK in Jurkat whole cell lysate.

      Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, 10μg
      Lane 2 (+): ab32507 & Jurkat whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab172638 in Jurkat whole cell lysate

      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Lane 1 : Anti-ITK antibody [Y402] (ab32507) at 1/2000 dilution (purified)
      Lane 2 : Anti-ITK antibody [Y402] (ab32507) at 1/10000 dilution (purified)

      All lanes : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 72 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Overlay histogram showing Jurkat cells stained with unpurified ab32507 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32507, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Anti-ITK antibody [Y402] (ab32507) at 1/1000 dilution (unpurified) + Jurkat cell lysate

      Predicted band size: 72 kDa
      Observed band size: 72 kDa

    文献

    This product has been referenced in:
    • Huck K  et al. Girls homozygous for an IL-2-inducible T cell kinase mutation that leads to protein deficiency develop fatal EBV-associated lymphoproliferation. J Clin Invest 119:1350-8 (2009). Read more (PubMed: 19425169) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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