Anti-ITIH4抗体(ab92338)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to ITIH4
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Orangutan
  • 免疫原

    Synthetic peptide:

    ERLCVDPRHRQGPVN

    , corresponding to amino acids 744-758 of Human ITIH4 (NP_002209.2.).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab92338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    May be involved in acute phase reactions.
  • 组织特异性
    Liver specific.
  • 序列相似性
    Belongs to the ITIH family.
    Contains 1 VIT domain.
    Contains 1 VWFA domain.
  • 翻译后修饰
    Cleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment.
    Appears to be both N- and O-glycosylated.
    The O-linked polysaccharides on Ser-696, Thr-701 and Ser-702 are probably the mucin type linked to GalNAc.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 antibody
    • gp120 antibody
    • H4P antibody
    • IHRP antibody
    • Inter-alpha-inhibitor heavy chain 4 antibody
    • Inter-alpha-trypsin inhibitor family heavy chain-related protein antibody
    • Inter-alpha-trypsin inhibitor heavy chain H4 antibody
    • inter-alpha-trypsin inhibitor, heavy chain-like, 1 antibody
    • ITI heavy chain H4 antibody
    • ITI-HC4 antibody
    • ITIH4 antibody
    • ITIH4_HUMAN antibody
    • ITIHL1 antibody
    • OTTHUMP00000197120 antibody
    • OTTHUMP00000197121 antibody
    • OTTHUMP00000213834 antibody
    • OTTHUMP00000213869 antibody
    • PK-120 antibody
    • PK120 antibody
    • Plasma kallikrein sensitive glycoprotein 120 antibody
    • PRO1851 antibody
    see all

Anti-ITIH4 antibody 图像

  • ab92338 (3.8µg/ml) staining of paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-ITIH4 antibody (ab92338)参考文献

ab92338 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Yes the anti-ITIH4 antibody ab92338 has been tested on endogenous protein, on human liver in IHC-P and on human vitreous in Western Blot, see the Abreview we received: http://www.abcam.com/ITIH4-antibody-ab92338-re...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - other (Vitreous)
Loading amount
2 µg
Specification
Vitreous
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Oct 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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