Anti-ITIH4抗体(ab92338)

Abreviews (1)Q&A (1)
Customer reviews and FAQs

概述

  • 产品名称
    Anti-ITIH4抗体
    参阅全部 ITIH4 一抗
  • 描述
    山羊多克隆抗体to ITIH4
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Orangutan
  • 免疫原

    Synthetic peptide:

    ERLCVDPRHRQGPVN

    , corresponding to amino acids 744-758 of Human ITIH4 (NP_002209.2.).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab92338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    May be involved in acute phase reactions.
  • 组织特异性
    Liver specific.
  • 序列相似性
    Belongs to the ITIH family.
    Contains 1 VIT domain.
    Contains 1 VWFA domain.
  • 翻译后修饰
    Cleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment.
    Appears to be both N- and O-glycosylated.
    The O-linked polysaccharides on Ser-696, Thr-701 and Ser-702 are probably the mucin type linked to GalNAc.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
    • 别名
      • 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 antibody
      • gp120 antibody
      • H4P antibody
      • IHRP antibody
      • Inter-alpha-inhibitor heavy chain 4 antibody
      • Inter-alpha-trypsin inhibitor family heavy chain-related protein antibody
      • Inter-alpha-trypsin inhibitor heavy chain H4 antibody
      • inter-alpha-trypsin inhibitor, heavy chain-like, 1 antibody
      • ITI heavy chain H4 antibody
      • ITI-HC4 antibody
      • ITIH4 antibody
      • ITIH4_HUMAN antibody
      • ITIHL1 antibody
      • OTTHUMP00000197120 antibody
      • OTTHUMP00000197121 antibody
      • OTTHUMP00000213834 antibody
      • OTTHUMP00000213869 antibody
      • PK-120 antibody
      • PK120 antibody
      • Plasma kallikrein sensitive glycoprotein 120 antibody
      • PRO1851 antibody
      see all

    Anti-ITIH4 antibody 图像

    • Western blot - ITIH4 antibody (ab92338)
      Western blot - ITIH4 antibody (ab92338)

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody (ab92338)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody (ab92338)
      ab92338 (3.8µg/ml) staining of paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    Anti-ITIH4 antibody (ab92338)参考文献

    ab92338 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab92338？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    提交 ab92338 的 abreview 提交 ab92338 的问题

    2 Abreviews or Q&A

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    Votes: Highest First

    To dear sir,
    we wanna to buy the antibody of Ab92338, before then, we want to request some informations about this antibody. According to the date sheet, they showed the WB figure which detected with Ab92338. i just wanna to ask it can be deteced i...

    Read More

    Thank you for contacting us.

    Yes the anti-ITIH4 antibody ab92338 has been tested on endogenous protein, on human liver in IHC-P and on human vitreous in Western Blot, see the Abreview we received: http://www.abcam.com/ITIH4-antibody-ab92338-re...

    Read More

    Western blot abreview for Anti-ITIH4 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - other (Vitreous)
    Loading amount
    2 µg
    Specification
    Vitreous
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
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    提交于 Oct 06 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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