Anti-ITGA7抗体[3C12] (ab195959)
Key features and details
- Mouse monoclonal [3C12] to ITGA7
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-ITGA7抗体[3C12]
参阅全部 ITGA7 一抗 -
描述
小鼠单克隆抗体[3C12] to ITGA7 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Tissue, cells or virus corresponding to Mouse ITGA7. ab195959 was raised against Mouse myoblasts.
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阳性对照
- Human brain, cortex tissue; C2C12 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
3C12 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab195959于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use a concentration of 10 - 20 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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Flow Cyt
Use a concentration of 10 - 20 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation (By similarity). Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells. -
组织特异性
Isoforms containing segment A are predominantly expressed in skeletal muscle. Isoforms containing segment B are abundantly expressed in skeletal muscle, moderately in cardiac muscle, small intestine, colon, ovary and prostate and weakly in lung and testes. Isoforms containing segment X2D are expressed at low levels in fetal and adult skeletal muscle and in cardiac muscle, but are not detected in myoblasts and myotubes. In muscle fibers isoforms containing segment A and B are expressed at myotendinous and neuromuscular junctions; isoforms containing segment C are expressed at neuromuscular junctions and at extrasynaptic sites. Isoforms containing segments X1 or X2 or, at low levels, X1X2 are expressed in fetal and adult skeletal muscle (myoblasts and myotubes) and cardiac muscle. -
疾病相关
Defects in ITGA7 are the cause of muscular dystrophy congenital due to integrin alpha-7 deficiency (MDCI) [MIM:613204]. A form of congenital muscular dystrophy. Patients present at birth, or within the first few months of life, with hypotonia, muscle weakness and often with joint contractures. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
发展阶段
In renewing intestinal epithelium, expression of isoforms containing segment B correlates with the onset of enterocytic differentiation. -
翻译后修饰
ADP-ribosylated on at least two sites of the extracellular domain in skeletal myotubes.
A 70 kDa form is created by proteolytic cleavage. Cleavage is elevated during myogenic differentiation and the cleaved form enhances cell adhesion and spreading on laminin. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3679 Human
- Entrez Gene: 16404 Mouse
- Omim: 600536 Human
- SwissProt: Q13683 Human
- SwissProt: Q61738 Mouse
- Unigene: 524484 Human
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别名
- alpha 7 antibody
- FLJ25220 antibody
- Integrin alpha 7 antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab195959 被引用在 2 文献中.
- Kobayashi N et al. Integrin a7 and Extracellular Matrix Laminin 211 Interaction Promotes Proliferation of Acute Myeloid Leukemia Cells and Is Associated with Granulocytic Sarcoma. Cancers (Basel) 12:N/A (2020). PubMed: 32033262
- Engl T et al. Acquired resistance to temsirolimus is associated with integrin a7 driven chemotactic activity of renal cell carcinoma in vitro. Oncotarget 9:18747-18759 (2018). PubMed: 29721158