Anti-ITCH/AIP4 (phospho Y420)抗体(ab111488)

概述

  • 产品名称Anti-ITCH/AIP4 (phospho Y420)抗体
    参阅全部 ITCH/AIP4 一抗
  • 描述
    兔多克隆抗体to ITCH/AIP4 (phospho Y420)
  • 特异性ab111488 detects endogenous levels of ITCH/AIP4 only when phosphorylated at tyrosine 420 (Human: Tyr420; Mouse: Tyr381).
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from Human ITCH/AIP4 around the phosphorylation site of tyrosine 420 (F-I-YP-G-N).

  • 阳性对照
    • Human brain tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab111488 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways. Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteosomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages. Critical regulator of T helper (TH2) cytokine development through its ability to induce JUNB ubiquitination and degradation (By similarity). Ubiquitinates SNX9. Ubiquitinates CXCR4 and HGS/HRS and regulates sorting of CXCR4 to the degradative pathway. It is involved in the negative regulation of MAVS-dependent cellular antiviral responses. Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteosomal degradation. Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteosomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteosomal degradation of p15 BID. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination.
  • 组织特异性Widely expressed.
  • 通路Protein modification; protein ubiquitination.
  • 疾病相关Defects in ITCH are the cause of syndromic multisystem autoimmune disease (SMAD) [MIM:613385]. SMAD is characterized by organomegaly, failure to thrive, developmental delay, dysmorphic features and autoimmune inflammatory cell infiltration of the lungs, liver and gut.
  • 序列相似性Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 4 WW domains.
  • 翻译后修饰On T-cell activation, phosphorylation by the JNK cascade on serine and threonine residues surrounding the PRR domain accelerates the ubiquitination and degradation of JUN and JUNB. The increased ITCH catalytic activity due to phosphorylation by JNK1 may occur due to a conformational change disrupting the interaction between the PRR/WW motifs domain and the HECT domain and, thus exposing the HECT domain (By similarity). Phosphorylation by FYN reduces interaction with JUNB and negatively controls JUN ubiquitination and degradation.
    Ubiquitinated; autopolyubiquitination with 'Lys-63' linkages which does not lead to protein degradation.
  • 细胞定位Cell membrane. Cytoplasm. Nucleus. Associates with endocytic vesicles. May be recruited to exosomes by NDFIP1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADMFD antibody
    • AIF4 antibody
    • AIP4 antibody
    • Atrophin 1 interacting protein 4 antibody
    • Atrophin-1-interacting protein 4 antibody
    • dJ468O1.1 antibody
    • dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)) antibody
    • dJ468O1.1 atrophin 1 interacting protein 4 AIP4 antibody
    • E3 ubiquitin protein ligase Itchy homolog antibody
    • E3 ubiquitin-protein ligase Itchy homolog antibody
    • EC 6.3.2 antibody
    • Itch antibody
    • ITCH_HUMAN antibody
    • Itchy E3 ubiquitin protein ligase antibody
    • Itchy E3 ubiquitin protein ligase homolog antibody
    • Itchy E3 ubiquitin protein ligase homolog mouse antibody
    • Itchy E3 ubiquitin protein ligase, mouse, homolog of antibody
    • Itchy homolog E3 ubiquitin protein ligase antibody
    • Itchy mouse homolog E3 ubiquitin protein ligase antibody
    • NAPP1 antibody
    • NFE2 associated polypeptide 1 antibody
    • NFE2-associated polypeptide 1 antibody
    • Ubiquitin protein ligase ITCH antibody
    see all

Anti-ITCH/AIP4 (phospho Y420) antibody 图像

  • ab111488, at 1/50 dilution, staining ITCH/AIP4 in paraffin-embedded Human brain tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-ITCH/AIP4 (phospho Y420) antibody (ab111488)参考文献

ab111488 has not yet been referenced specifically in any publications.

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