Mouse, Rat, Human
Recombinant fragment corresponding to amino acids 23-146 of Human ISY1 (Q9ULR0).
Human cell line U-2 OS; RT-4 cells, U-251 MG cells and Human tonsil lysates; Human stomach tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/250 - 1/500.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
May play a role in pre-mRNA splicing.
Belongs to the ISY1 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
ISY1 splicing factor homolog (S. cerevisiae) antibody
Immunocytochemistry/ Immunofluorescence - Anti-ISY1 antibody (ab121250)
ICC/IF (Immunocytochemistry/immunofluorescence) of Human cell line U-2 OS labelling ISY1 (green) with ab121250 at 1µg/ml. Cells were fixed with PFA and permeabilized with Triton X-100. Immunofluorescent staining shows positivity in nucleus but not nucleoli.
Western blot - Anti-ISY1 antibody (ab121250)
All lanes : Anti-ISY1 antibody (ab121250) at 1/250 dilution Lane 1 : Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2 : RT-4 cell lysate Lane 3 : U-251 MG cell lysate Lane 4 : Human plasma lysate Lane 5 : Liver lysate Lane 6 : Tonsil lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISY1 antibody (ab121250)
Immunohistochemical staining of paraffin embedded Human stomach, using 1/75 ab121250, shows moderate nuclear positivity in glandular cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ISY1 antibody(ab121250)
Immunohistochemical staining of Human stomach shows moderate nuclear positivity in glandular cells using ab121250
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