Anti-Isoleucyl tRNA synthetase抗体(ab76971)

概述

  • 产品名称Anti-Isoleucyl tRNA synthetase抗体
    参阅全部 Isoleucyl tRNA synthetase 一抗
  • 描述
    小鼠单克隆抗体to Isoleucyl tRNA synthetase
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Recombinant fragment: QYINLQLLNA KPQECLMGTV GTLLLENPLG QNGLTHQGLL YEAAKVFGLR SRKLKLFLNE TQTQEITEDI PVKTLNMKTV YVSVLPTTAD F, corresponding to amino acids 1172-1262 of human Isoleucyl tRNA synthetase (NP_038203) with a 26 kDa tag.

  • 阳性对照
    • Isoleucyl tRNA synthetase transfected 293T cell lysate; immunogen

性能

应用

Our Abpromise guarantee covers the use of ab76971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 145 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性Isoleucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of isoleucine with isoleucyl tRNA.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • FLJ20736 antibody
    • IARS antibody
    • IARS1 antibody
    • IleRS antibody
    • ILRS antibody
    • IRS antibody
    • Isoleucine tRNA ligase 1 cytoplasmic antibody
    • Isoleucine tRNA ligase antibody
    • Isoleucine tRNA synthetase antibody
    • Isoleucyl tRNA synthetase antibody
    • PRO0785 antibody
    see all

Anti-Isoleucyl tRNA synthetase antibody 图像

  • All lanes : Anti-Isoleucyl tRNA synthetase antibody (ab76971) at 5 µg/ml

    Lane 1 : Isoleucyl tRNA synthetase transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 145 kDa
    Observed band size : 121 kDa (why is the actual band size different from the predicted?)
  • Anti-Isoleucyl tRNA synthetase antibody (ab76971) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 145 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

Anti-Isoleucyl tRNA synthetase antibody (ab76971)参考文献

ab76971 has not yet been referenced specifically in any publications.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"