Anti-Isocitrate dehydrogenase抗体(ab85532)

概述

  • 产品名称Anti-Isocitrate dehydrogenase抗体
    参阅全部 Isocitrate dehydrogenase 一抗
  • 描述
    兔多克隆抗体to Isocitrate dehydrogenase
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human, Pig
    预测可用于: Mouse
  • 免疫原

    Purified Isocitrate dehydrogenase from Pig heart.

  • 阳性对照
    • Heart tissue.

性能

应用

Our Abpromise guarantee covers the use of ab85532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
ELISA 1/400 - 1/1600.
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 疾病相关Glioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
  • 序列相似性Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • 翻译后修饰Acetylation at Lys-374 dramatically reduces catalytic activity.
  • 细胞定位Cytoplasm. Peroxisome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytosolic NADP isocitrate dehydrogenase antibody
    • Cytosolic NADP-isocitrate dehydrogenase antibody
    • Epididymis luminal protein 216 antibody
    • Epididymis secretory protein Li 26 antibody
    • HEL-216 antibody
    • HEL-S-26 antibody
    • ICDH antibody
    • IDCD antibody
    • IDH antibody
    • IDH1 antibody
    • IDHC_HUMAN antibody
    • IDP antibody
    • IDPC antibody
    • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
    • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
    • NADP dependent isocitrate dehydrogenase cytosolic antibody
    • NADP dependent isocitrate dehydrogenase peroxisomal antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    • PICD antibody
    see all

Anti-Isocitrate dehydrogenase antibody 图像

  • ab85532 at 10µg/ml staining Isocitrate dehydrogenase in human heart tissue, by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue

Anti-Isocitrate dehydrogenase antibody (ab85532)参考文献

ab85532 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

As I understand it, if the recommended IHC working concentration for this antibody is 10ug/ml, and 100ul are needed for slide, then you would need 1ug of antibody per 100ul of diluent, that is, 1ug of antibody per...

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Thank you for contacting us. I would say that this sort of experiment is very-very tricky. We unfortunately do not have any protocol available at the moment for manually IHC staining quantification with antibodies. I would suggest ploughing through...

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Thank you for your enquiry. This antibody was raised against isocytrate dehydrogenase purified from porcine heart. According to our collaborator who developed the antibody, they have only tested it against wild type isocytrate dehydrogenase. It h...

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