antigen sequence: QVTIKVVEDP QAEVSIDLLA EPSNPPPQDT LSWLPALWSF LWGDYKGEEK DRAPGEKGEE KEEDEDYPSE DIEGEDQE, corresponding to internal sequence amino acids 209-286 of Human ISM2.
- Human smooth muscle tissue; U 251 MG cells.
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
Expressed at high levels in the placenta and at moderate levels in the pancreas, kidney, heart, liver, lung, brain and skeletal muscle.
Belongs to the isthmin family.
Contains 1 AMOP domain.
Contains 1 TSP type-1 domain.
Information by UniProt
- Gm264 antibody
- ISM2 antibody
- ISM2_HUMAN antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISM2 antibody (ab122582)
ab122582, at 1/20 dilution, staining ISM2 in paraffin-embedded Human smooth muscle tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - (ab122582)
ab122582, at 4 µg/ml, staining ISM2 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
has not yet been referenced specifically in any publications.
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