概述

  • 产品名称Anti-Islet 2抗体
    参阅全部 Islet 2 一抗
  • 描述
    小鼠单克隆抗体to Islet 2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 261-359 of Human Islet 2 (NP_665804)

  • 阳性对照
    • Islet 2 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab77212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ELISA Use at an assay dependent dilution.

靶标

  • 功能Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.
  • 序列相似性Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ10160 antibody
    • Insulin gene enhancer protein ISL 2 antibody
    • Insulin gene enhancer protein ISL-2 antibody
    • Insulin gene enhancer protein ISL2 antibody
    • ISL 2 antibody
    • ISL 2 transcription factor antibody
    • ISL 2 transcription factor LIM/homeodomain antibody
    • ISL LIM homeobox 2 antibody
    • ISL2 antibody
    • ISL2 transcription factor antibody
    • ISL2 transcription factor LIM/homeodomain antibody
    • ISL2_HUMAN antibody
    • Islet-2 antibody
    • Islet2 antibody
    • SL2 transcription factor LIM/homeodomain (islet-2) antibody
    see all

Anti-Islet 2 antibody 图像

  • Anti-Islet 2 antibody (ab77212) at 5 µg/ml + Recombinant fragment of human Islet 2 at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 40 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Islet 2 antibody (ab77212) at 5 µg/ml

    Lane 1 : Islet 2 transfected lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 40 kDa
    Observed band size : 40 kDa

Anti-Islet 2 antibody (ab77212)参考文献

ab77212 has not yet been referenced specifically in any publications.

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