Anti-Islet 1抗体- Neural Stem Cell Marker (ab20670)

概述

  • 产品名称
    Anti-Islet 1抗体- Neural Stem Cell Marker
    参阅全部 Islet 1 一抗
  • 描述
    兔多克隆抗体to Islet 1 - Neural Stem Cell Marker
  • 特异性
    ab20670 might also detect rat and human Islet 2 protein as the immunogen used to raise this ab20670 is 86% identical to rat and human Islet 2. This has not been tested.
  • 经测试应用
    适用于: IHC-FoFr, IHC-P, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Apteronotus leptorhynchus
    预测可用于: Chicken, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Islet 1.

    (Peptide available as ab21996.)

  • 阳性对照
    • IHC-P: FFPE mouse embryo E12.

性能

应用

Our Abpromise guarantee covers the use of ab20670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 20096094
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 2 µg/ml.
IHC-Fr 1/500.

靶标

Anti-Islet 1 antibody - Neural Stem Cell Marker 图像

  • IHC image of Islet 1 staining in a section of formalin fixed, paraffin embedded mouse embryo E12, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab20670, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of Islet 1 staining in a section of formalin fixed, paraffin embedded mouse embryo E12, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab20670, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab20670 2µg/ml staining ISLET1 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and weak cytoplasmic staining primarily in the pancreatic islet.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Dorsal root ganglion explants were dissected from 16 day-old rat embryos and cultured for 6 hours in vitro with Neurobasal Medium containing B27 supplement.     

    Nuclei stained positive for anti-Islet 1 antibody ab20670 at 2µg/ml. As would be expected, not all cells in this preparation were Islet 1-positive. Pre-incubation of ab20670 with the immunizing peptide ab21996 resulted in complete blocking of the antibody.

    Green = ab20670
    Blue = To-pro-3 nuclear stain

    The level of magnification is different in each image.    

  • Immunocytochemistry/ Immunofluorescence analysis of adult mouse cardiac fibroblasts labeling Islet 1 with ab20670 at 1/100 dilution (green). Samples were fixed using 4% PFA with 0.25% TritonX-100. Scale bar is 100 µM.

  • Immunohistochemistry (Frozen sections) analysis of mouse brain tissue sections labeling Islet 1 with ab20670 at 1/200 dilution. The tissue was fixed with paraformaldehyde followed by blocking with 5% serum for 1 hour at 25°C. The tissue was incubated with ab20670 in PBST for 12 hours at 4°C. A polyclonal donkey anti-rabbit Alexa Fluor® 594 secondary antibody was used at 1/1000 dilution.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence analysis of mouse dorsal root ganglia (DRG) neurons labeling Islet 1 with ab20670 at 1/200 dilution. Cells were fixed with paraformaldehyde and permeabilized with Tween 20. Blocking of the cells was performed with 2% serum for 30 minutes at 21°C, followed by incubation with ab20670 for 18 hours at 4°C. A polyclonal goat anti-rabbit Alexa Fluor® 568 secondary antibody was used at 1/1000 dilution. DAPI was used to counterstain.

    See Abreview

  • Immunohistochemical analysis of paraffin-embedded mouse E11.5 somites labelling Islet 1 with ab20670 at 1/100 dilution, followed by BioGenex polymer-HRP reagent (undiluted according to manufacturer).

  • ab20670 detected Islet 1 in the nucleus of frozen sections of E11.5 mouse embryonic brain using 1 ug/ml of antibody. Brighter staining may be achieved using a greater concentration of antibody, e.g. 2.5 or 5 ug/ml.

Anti-Islet 1 antibody - Neural Stem Cell Marker (ab20670)参考文献

This product has been referenced in:
  • Nishi K  et al. Nardilysin Is Required for Maintaining Pancreatic ß-Cell Function. Diabetes 65:3015-27 (2016). Read more (PubMed: 27385158) »
  • Synnergren J  et al. Comparative transcriptomic analysis identifies genes differentially expressed in human epicardial progenitors and hiPSC-derived cardiac progenitors. Physiol Genomics 48:771-784 (2016). ICC/IF ; Human . Read more (PubMed: 27591124) »

See all 24 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cell (Differentiated mES cell)
Permeabilization
Yes - 0.2% Triton X
Specification
Differentiated mES cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 23 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification
Human pluripotent stem cell derived cardiomyocyte
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 12 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated
Sample
Spotted Catshark (Scyliorhinus canicula) Tissue sections (brain cells)
Specification
brain cells
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Liam Rasch

Verified customer

提交于 Nov 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
Specification
Dorsal Root Ganglia (DRG) neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - tween 20
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Oct 18 2012

Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 28 2012

Thank you for your message.

I am pleased the results have improved. In order to help further, I would appreciate if you are able to send an attached image which will help me to assess the results.

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Thank you for contacting Abcam.

Please find attached the Certificate of Compliance for ab20670, lot #*.

If there is anything else I can help you with, please let me know.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Adult heart)
Specification
Adult heart
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Tx100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jun 19 2012

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