Anti-Islet 1抗体[1H9] (ab86472)
Key features and details
- Mouse monoclonal [1H9] to Islet 1
- Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-Islet 1抗体[1H9]
参阅全部 Islet 1 一抗 -
描述
小鼠单克隆抗体[1H9] to Islet 1 -
宿主
Mouse -
特异性
In western blot, we observe a specific band at ~40kDa which is not seen in KO cell lines. A cross-reactive band was observed in the wild-type and knockout cells. The additional band below this band of interest is seen at ~38kDa in both the WT and KO cells. We are unsure as to the identity of these extra bands. -
经测试应用
适用于: Flow Cyt, WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human Islet 1 aa 150-349. Purified recombinant fragment of Human Islet 1 expressed in E. Coli
Database link: P61371 -
阳性对照
- Transfected HEK293 cell lysate. Human lung cancer tissue and cervical carcinoma tissue.
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常规说明
This product was changed from ascites to supernatant. Lot no’s high than GR141006-29 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
1H9 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86472于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
1/50 - 1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB |
1/500 - 1/2000. Predicted molecular weight: 39 kDa.
|
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IHC-P |
1/200 - 1/1000.
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ICC/IF |
1/200 - 1/1000.
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说明 |
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Flow Cyt
1/50 - 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 39 kDa. |
IHC-P
1/200 - 1/1000. |
ICC/IF
1/200 - 1/1000. |
靶标
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功能
Binds to one of the cis-acting domain of the insulin gene enhancer. -
组织特异性
Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. -
序列相似性
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3670 Human
- Omim: 600366 Human
- SwissProt: P61371 Human
- Unigene: 505 Human
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别名
- Insulin gene enhancer protein ISL 1 antibody
- Insulin gene enhancer protein ISL-1 antibody
- Insulin related protein antibody
see all
图片
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ab86472 at a 1/200 dilution staining Islet 1 in human lung cancer tissue. DAB staining shows nuclear localisation.
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All lanes : Anti-Islet 1 antibody [1H9] (ab86472) at 1/500 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ISL1 (Islet 1) knockout HAP1 whole cell lysate
Lane 3 : SH-SY5Y whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab86472 observed at 40 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab86472 was shown to recognize Islet 1 in wild-type HAP1 cells as signal was lost at the expected MW in Islet 1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Islet 1 knockout samples were subjected to SDS-PAGE. ab86472 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Anti-Islet 1 antibody [1H9] (ab86472) at 1/500 dilution + HEK293 cells transfected with full length Islet 1 (aa1-349)-hIgGFc
Predicted band size: 39 kDaNote: The molecular weight is larger than expected, as the recombinant protein is not the endogenouse protein, but rather the fc-fusion protein
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Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length Islet 1-hIgGFc using ab86472 at 1/150 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
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ab86472 at a 1/200 dilution staining Islet 1 in human cervical carcinoma tissue. DAB staining shows nuclear localization.
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Overlay histogram showing SH-SY5Y cells stained with ab86472 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86472, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab86472 被引用在 17 文献中.
- Bauer US et al. Validation of Functional Connectivity of Engineered Neuromuscular Junction With Recombinant Monosynaptic Pseudotyped ΔG-Rabies Virus Tracing. Front Integr Neurosci 16:855071 (2022). PubMed: 35669734
- Moyon S et al. TET1-mediated DNA hydroxymethylation regulates adult remyelination in mice. Nat Commun 12:3359 (2021). PubMed: 34099715
- Mahmoodi N et al. Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel. Sci Rep 11:21722 (2021). PubMed: 34741076
- Xu J et al. Therapeutic effects of CXCR4+ subpopulation of transgene-free induced cardiosphere-derived cells on experimental myocardial infarction. Cell Prolif 54:e13041 (2021). PubMed: 33942933
- Akhir MKAM et al. The Role of ISL1 and LHX5 LIM Homeobox Genes in Bladder Tumourigenesis. Malays J Med Sci 27:37-45 (2020). PubMed: 32158343