Anti-Islet 1抗体[1H9] (ab86472)

概述

  • 产品名称Anti-Islet 1抗体[1H9]
    参阅全部 Islet 1 一抗
  • 描述
    小鼠单克隆抗体[1H9] to Islet 1
  • 经测试应用适用于: Flow Cyt, WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human Islet 1 aa 150-349. Purified recombinant fragment of Human Islet 1 expressed in E. Coli
    Database link: P61371

  • 阳性对照
    • Transfected HEK293 cell lysate. Human lung cancer tissue and cervical carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab86472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Predicted molecular weight: 39 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.

靶标

  • 功能Binds to one of the cis-acting domain of the insulin gene enhancer.
  • 组织特异性Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
  • 序列相似性Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Insulin gene enhancer protein ISL 1 antibody
    • Insulin gene enhancer protein ISL-1 antibody
    • Insulin related protein antibody
    • ISL 1 antibody
    • ISL LIM homeobox 1 antibody
    • ISL1 antibody
    • ISL1 transcription factor LIM homeodomain antibody
    • ISL1 transcription factor, LIM/homeodomain (islet 1) antibody
    • ISL1 transcription factor, LIM/homeodomain antibody
    • ISL1_HUMAN antibody
    • Islet-1 antibody
    • Islet1 antibody
    see all

Anti-Islet 1 antibody [1H9] 图像

  • Anti-Islet 1 antibody [1H9] (ab86472) at 1/500 dilution + HEK293 cells transfected with full length Islet 1 (aa1-349)-hIgGFc

    Predicted band size : 39 kDa

    Note: The molecular weight is larger than expected, as the recombinant protein is not the endogenouse protein, but rather the fc-fusion protein

  • Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length Islet 1-hIgGFc using ab86472 at 1/150 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

  • ab86472 at a 1/200 dilution staining Islet 1 in human lung cancer tissue. DAB staining shows nuclear localisation.
  • ab86472 at a 1/200 dilution staining Islet 1 in human cervical carcinoma tissue. DAB staining shows nuclear localization.
  • Overlay histogram showing SH-SY5Y cells stained with ab86472 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86472, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Islet 1 antibody [1H9] (ab86472)参考文献

This product has been referenced in:
  • Zhang Q  et al. ISL-1 is overexpressed in non-Hodgkin lymphoma and promotes lymphoma cell proliferation by forming a p-STAT3/p-c-Jun/ISL-1 complex. Mol Cancer 13:181 (2014). IHC-P ; Human . Read more (PubMed: 25070240) »
  • Fonoudi H  et al. ISL1 Protein Transduction Promotes Cardiomyocyte Differentiation from Human Embryonic Stem Cells. PLoS One 8:e55577 (2013). WB ; Human . Read more (PubMed: 23383231) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 37°C
Sample Dog Tissue sections (Heart)
Specification Heart
Permeabilization Yes - 0.5% Tween80 PBS wash
Fixative Paraformaldehyde
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Verified customer

提交于 Oct 13 2014

Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it...

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Thank you for contacting Abcam regarding ab86472. I am sorry that you are experiencing difficulties with some lots of this antibody in IHC. I have checked our current stock and currently we have a new lot available. I would be happy to send this as...

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Vielen Dank fuer Ihre email. Es tut mir sehr leid zu hoeren, dass das aktuelle Lot fuer ab86472 Ihnen Probleme bereitet. Eine Ersatzlieferung waere auf jeden Fall moeglich, falls der Antikoerper in getesteten Anwendungen (ELISA, ICC/IF, IHC-P, W...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"