Anti-Islet 1抗体[1B1] (ab86501)


  • 产品名称Anti-Islet 1抗体[1B1]
    参阅全部 Islet 1 一抗
  • 描述
    小鼠单克隆抗体[1B1] to Islet 1
  • 经测试应用适用于: Flow Cyt, IHC-P, WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human Islet 1 aa 150-349. Purified recombinant fragment expressed in E. Coli.
    Database link: P61371

  • 阳性对照
    • WB: Full length Islet 1 (aa 1-349)-hIgGFc transfected HEK293 cell lysate. ICC/IF: HEK293 cells transfected with full length Islet 1-hIgGFc. IHC-P (FFPE): Human Pancreas (Normal) tissue.



Our Abpromise guarantee covers the use of ab86501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/2000. Predicted molecular weight: 39 kDa.
ELISA 1/10000.
ICC/IF 1/200 - 1/1000.


  • 功能Binds to one of the cis-acting domain of the insulin gene enhancer.
  • 组织特异性Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
  • 序列相似性Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Insulin gene enhancer protein ISL 1 antibody
    • Insulin gene enhancer protein ISL-1 antibody
    • Insulin related protein antibody
    • ISL 1 antibody
    • ISL LIM homeobox 1 antibody
    • ISL1 antibody
    • ISL1 transcription factor LIM homeodomain antibody
    • ISL1 transcription factor, LIM/homeodomain (islet 1) antibody
    • ISL1 transcription factor, LIM/homeodomain antibody
    • ISL1_HUMAN antibody
    • Islet-1 antibody
    • Islet1 antibody
    see all

Anti-Islet 1 antibody [1B1] 图像

  • Anti-Islet 1 antibody [1B1] (ab86501) at 1/500 dilution + full length Islet 1-hIgGFc transfected HEK293 cell lysate

    Predicted band size : 39 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Note: The molecular weight is larger than expected, as the recombinant protein is not the endogenouse protein, but rather the fc-fusion protein.
  • Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length Islet 1-hIgGFc using ab86501 at 1/150 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

  • IHC image of Islet 1 staining in Human Pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86501, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Overlay histogram showing SH-SY5Y cells stained with ab86501 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86501, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.


Anti-Islet 1 antibody [1B1] (ab86501)参考文献

ab86501 has not yet been referenced specifically in any publications.

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