Anti-ISG20抗体(ab50834)

概述

  • 产品名称Anti-ISG20抗体
    参阅全部 ISG20 一抗
  • 描述
    兔多克隆抗体to ISG20
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide: VLYDKFIRPE GEITDYRTRV SGVTPQHMVG ATPFAVARLE ILQLLKGKLV , corresponding to amino acids 37-86 of Human ISG20

  • 阳性对照
    • Daudi Cell Lysate

性能

应用

Our Abpromise guarantee covers the use of ab50834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay is 1:1562500.

IHC-P Use at an assay dependent concentration.

靶标

  • 功能Exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. Degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. Involved in the antiviral function of IFN against RNA viruses.
  • 组织特异性Highly expressed in peripheral blood leukocytes, spleen, thymus, colon and lung.
  • 序列相似性Belongs to the exonuclease superfamily.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD25 antibody
    • Estrogen regulated transcript 45 protein antibody
    • Estrogen-regulated transcript 45 protein antibody
    • HEM45 antibody
    • Interferon stimulated exonuclease gene 20kDa antibody
    • Interferon stimulated gene 20 kDa protein antibody
    • Interferon-stimulated gene 20 kDa protein antibody
    • Isg20 antibody
    • ISG20_HUMAN antibody
    • Promyelocytic leukemia nuclear body associated protein antibody
    • Promyelocytic leukemia nuclear body-associated protein ISG20 antibody
    see all

Anti-ISG20 antibody 图像

  • Anti-ISG20 antibody (ab50834) at 1.25 µg/ml + Daudi Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 50000

    Predicted band size : 20 kDa
    Observed band size : 20 kDa

Anti-ISG20 antibody (ab50834)参考文献

ab50834 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Raw 264.7 cells and THP1 cells)
Gel Running Conditions Reduced Denaturing (12% gel (Acrylamide))
Loading amount 20 µg
Treatment Interferon bete (2h, 12h, 24h)
Specification Raw 264.7 cells and THP1 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Mar 17 2016

I am very pleased to hear you would like to accept our offer and test ab50834 in mouse. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the A...

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Thank you for contacting Abcam regarding ab50834.


We have not yet received feedback on how this antibody works in mouse. If you are interested in testing this yourself, I am happy to offer the same testing discount as this customer had re...

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I am very pleased to hear you would like to accept our offer and test ab50834 in mouse. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the &l...

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Thank you very much for your interest in ab50834 and ab55338.

To our knowledge, neither antibody has been tested in mouse.



We do not have trial sizes of our products. Therefore, I can offer a discount off a future purcha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"