The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
1/2000 - 1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
1/100 - 1/250.
应用说明Is unsuitable for Flow Cyt or IP.
功能Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
组织特异性Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
序列相似性Contains 2 ubiquitin-like domains.
细胞定位Cytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, staining ISG15 with ab92345 at 1/100 dilution.
Western blot - ISG15 antibody [EPR3445] (ab92345)
All lanes : Anti-ISG15 antibody [EPR3445] (ab92345) at 1/2000 dilution
Lane 1 : Hela cell lysate treated with IFN α Lane 2 : Daudi cell lysate
Lysates/proteins at 10 µg per lane.
Secondary goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 15 kDa Observed band size : 15 kDa
Anti-ISG15 antibody [EPR3445] (ab92345)参考文献
This product has been referenced in:
Kim K et al. Reinvestigation of aminoacyl-TRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex. PLoS One8:e81734 (2013).
Read more (PubMed: 24312579) »
Pal S et al. Influenza A virus interacts extensively with the cellular SUMOylation system during infection. Virus Res158:12-27 (2011).
Read more (PubMed: 21376763) »