概述

  • 产品名称Anti-ISG15抗体
    参阅全部 ISG15 一抗
  • 描述
    兔多克隆抗体to ISG15
  • 经测试应用适用于: WB, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant ISG15(Human).

  • 阳性对照
    • Most human cell lysates can be used as a positive control without induction or stimulation.
  • 常规说明Centrifuge product if not completely clear after standing at room temperature.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit serum.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab14374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/1000. Detects a band of approximately 18.5 kDa.
ELISA 1/2000 - 1/10000.
IHC-Fr Use at an assay dependent concentration. PubMed: 20186858

靶标

  • 功能Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.
    In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
  • 组织特异性Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
  • 序列相似性Contains 2 ubiquitin-like domains.
  • 细胞定位Cytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • G1P2 antibody
    • hUCRP antibody
    • IFI 15 antibody
    • IFI15 antibody
    • Interferon alpha inducible protein antibody
    • Interferon induced protein 15 antibody
    • Interferon induced protein IFI-15K antibody
    • Interferon stimulated protein 15 kDa antibody
    • Interferon, alpha-inducible protein (clone IFI-15K) antibody
    • Interferon-induced 15 kDa protein antibody
    • Interferon-induced 17 kDa protein antibody
    • Interferon-induced 17-kDa/15-kDa protein antibody
    • IP17 antibody
    • ISG 15 antibody
    • ISG15 antibody
    • ISG15 ubiquitin like modifier antibody
    • ISG15_HUMAN antibody
    • Ubiquitin cross-reactive protein antibody
    • Ubiquitin-like protein ISG15 antibody
    • UCRP antibody
    see all

Anti-ISG15 antibody 图像

  • Anti-ISG15 antibody (ab14374) at 1/500 dilution + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 12 minutes

Anti-ISG15 antibody (ab14374)参考文献

This product has been referenced in:
  • Hirashima K  et al. Telomere length influences cancer cell differentiation in vivo. Mol Cell Biol 33:2988-95 (2013). ICC/IF . Read more (PubMed: 23716593) »
  • Salajegheh M  et al. Interferon-stimulated gene 15 (ISG15) conjugates proteins in dermatomyositis muscle with perifascicular atrophy. Ann Neurol 67:53-63 (2010). IHC-Fr ; Human . Read more (PubMed: 20186858) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry. No, I do not know of anyone that did not see these extra bands. It is expected to see these bands. My understanding is that these extra bands come from the binding of other proteins to ISG15 and that these proteins are ...

Read More

Thank you for your phone call. Yes, ab14374 and ab14687 are the same antibody. I have removed ab14687 from our list and so you can go ahead and order ab14374. Please let me know if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"