概述

  • 产品名称Anti-IRS4抗体[EP907Y]
    参阅全部 IRS4 一抗
  • 描述
    兔单克隆抗体[EP907Y] to IRS4
  • 经测试应用适用于: IHC-P, WB, IP, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues from the C terminus of human IRS4.

  • 阳性对照
    • Hek293 cell lysate and Human kidney tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab52622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
IP 1/20.
Flow Cyt 1/20 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性IRS4 (Insulin receptor substrate 4) is a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signaling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.
  • 细胞定位Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • 数据库链接
  • 别名
    • 160 kDa phosphotyrosine protein antibody
    • Insulin receptor substrate 4 antibody
    • IRS 4 antibody
    • PY160 antibody

Anti-IRS4 antibody [EP907Y] 图像

  • Anti-IRS4 antibody [EP907Y] (ab52622) at 1/5000 dilution + Hek293 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 133 kDa
    Observed band size : 133 kDa
  • ab52622, at a 1/50 dilution staining Human IRS4 in kidney using Immunohistochemistry, Paraffin embedded tissue.
  • Overlay histogram showing HEK293 cells stained with ab52622 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52622, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-IRS4 antibody [EP907Y] (ab52622)参考文献

This product has been referenced in:
  • Homma Y  et al. Insulin receptor substrate-4 binds to Slingshot-1 phosphatase and promotes cofilin dephosphorylation. J Biol Chem 289:26302-13 (2014). Read more (PubMed: 25100728) »
  • Chai B  et al. A novel transcript is up-regulated by fasting in the hypothalamus and enhances insulin signaling. J Neuroendocrinol : (2012). Read more (PubMed: 22935015) »

See all 4 Publications for this product

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