RabMAb

Anti-IRS2抗体[EP976Y] (ab52606)

概述

  • 产品名称Anti-IRS2抗体[EP976Y]
    参阅全部 IRS2 一抗
  • 描述
    兔单克隆抗体[EP976Y] to IRS2
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues surrounding Ser1203 of human IRS2.

  • 阳性对照
    • 293 cell lysate; human kidney tissue. IF/ICC: SKNSH cell line.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab52606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100000. Detects a band of approximately 185 kDa (predicted molecular weight: 137 kDa).
IP 1/50.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能May mediate the control of various cellular processes by insulin.
    • 序列相似性Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Cytoplasm > cytosol.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Insulin receptor substrate 2 antibody
      • IRS 2 antibody
      • IRS-2 antibody
      • Irs2 antibody
      • IRS2_HUMAN antibody
      see all

    Anti-IRS2 antibody [EP976Y] 图像

    • ab52606 stained SKNSH cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52606 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Anti-IRS2 antibody [EP976Y] (ab52606) at 1/100000 dilution + 293 cell lysate at 10 µg

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 137 kDa
      Observed band size : 185 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of paraffin-embedded human kidney using ab52606 at a dilution of 1/100-1/250.

    Anti-IRS2 antibody [EP976Y] (ab52606)参考文献

    ab52606 has not yet been referenced specifically in any publications.

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