Anti-IRS2 antibody [EP903Y] (ab46811) at 1/100000 dilution + 293 cell lysate at 10ug/lane, plus insulin
Secondary Goat-anti rabbit, HRP labelled, at 1/2000 dilution
Predicted band size : 137 kDa Observed band size : 160 kDa (why is the actual band size different from the predicted?) Additional bands at : 50 kDa (possible non-specific binding).The 50 kDa band is most likely the heavy chain. This appears when using human tissue or samples which may contain traces of blood. There are secondary antibodies such as Tru-Blot that can eliminate this heavy chain band.
This image shows human cervical carcinoma stained with ab46811 at 1/50 dilution.
This product has been referenced in:
Lopez R et al. Hyperglycemia enhances the proliferation of non-tumorigenic and malignant mammary epithelial cells through increased leptin/IGF1R signaling and activation of AKT/mTOR. PLoS One8:e79708 (2013).
Read more (PubMed: 24260287) »