概述

  • 产品名称Anti-IRS2抗体[EP903Y]
    参阅全部 IRS2 一抗
  • 描述
    兔单克隆抗体[EP903Y] to IRS2
  • 经测试应用适用于: WB, IHC-P, ICC/IF, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human IRS2.

  • 阳性对照
    • 293 cell lysate + insulin; human cervical carcinoma tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab46811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100000. Predicted molecular weight: 137 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
IP 1/50.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能May mediate the control of various cellular processes by insulin.
    • 序列相似性Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Cytoplasm > cytosol.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Insulin receptor substrate 2 antibody
      • IRS 2 antibody
      • IRS-2 antibody
      • Irs2 antibody
      • IRS2_HUMAN antibody
      see all

    Anti-IRS2 antibody [EP903Y] 图像

    • Anti-IRS2 antibody [EP903Y] (ab46811) at 1/100000 dilution + 293 cell lysate at 10ug/lane, plus insulin

      Secondary
      Goat-anti rabbit, HRP labelled, at 1/2000 dilution

      Predicted band size : 137 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa (possible non-specific binding).
      The 50 kDa band is most likely the heavy chain. This appears when using human tissue or samples which may contain traces of blood. There are secondary antibodies such as Tru-Blot that can eliminate this heavy chain band.
    • This image shows human cervical carcinoma stained with ab46811 at 1/50 dilution.

    Anti-IRS2 antibody [EP903Y] (ab46811)参考文献

    This product has been referenced in:
    • Lopez R  et al. Hyperglycemia enhances the proliferation of non-tumorigenic and malignant mammary epithelial cells through increased leptin/IGF1R signaling and activation of AKT/mTOR. PLoS One 8:e79708 (2013). WB ; Human . Read more (PubMed: 24260287) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"