概述

  • 产品名称Anti-IRS1 (phospho Y612)抗体
    参阅全部 IRS1 一抗
  • 描述
    兔多克隆抗体to IRS1 (phospho Y612)
  • 特异性Cross-reactivty with IRS2 has not been tested. Immunogen for ab66153 has 75% homology with corresponding IRS2 sequence.
  • 经测试应用适用于: IHC-P, WB, IP, Indirect ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to the epitope DGYMP with a single phosphorylation site, Tyr612, from human IRS1.

  • 阳性对照
    • Insulin stimulated CHO cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab66153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 165 kDa (predicted molecular weight: 132 kDa).
IP Use a concentration of 2 - 5 µg/ml.
Indirect ELISA Use a concentration of 0.01 - 0.1 µg/ml.

靶标

  • 功能May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • 疾病相关Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • 序列相似性Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 翻译后修饰Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Anti-IRS1 (phospho Y612) antibody 图像

  • Immunoprecipitation analysis of insulin stimulated CHO cells precipitating IRS1 (phospho Y912) with ab66153. Western blot was performed with ab66153 at 1:500 (Lane 1). Lane 2 is a negative control.

  • 1µg of peptide was placed on a nitrocellulose membrane.

    Peptides: A: IRS1(pY612) B: IRS1 (non phosphorylated) C: Non-related Phosphopeptide.

    Lane 1: ab66153 at 1/1000. Lane 2: Rabbit anti-non-pY612 IRS1 at 1/1000.

Anti-IRS1 (phospho Y612) antibody (ab66153)参考文献

This product has been referenced in:
  • Moghei M  et al. Ketoisocaproic acid, a metabolite of leucine, suppresses insulin-stimulated glucose transport in skeletal muscle cells in a BCAT2-dependent manner. Am J Physiol Cell Physiol 311:C518-27 (2016). Read more (PubMed: 27488662) »
  • Pan T  et al. Cytohesins/ARNO: the function in colorectal cancer cells. PLoS One 9:e90997 (2014). Read more (PubMed: 24618737) »

See all 6 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab66153.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"