Anti-IRS1 (phospho S616)抗体(ab58479)

概述

  • 产品名称Anti-IRS1 (phospho S616)抗体
    参阅全部 IRS1 一抗
  • 描述
    兔多克隆抗体to IRS1 (phospho S616)
  • 特异性ab58479 detects endogenous levels of IRS1 only when phosphorylated at serine 612.
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human IRS1.

  • 阳性对照
    • Human breast carcinoma tissue; HUVEC lysate.

性能

应用

Our Abpromise guarantee covers the use of ab58479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 131 kDa.

靶标

  • 功能May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • 疾病相关Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • 序列相似性Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 翻译后修饰Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Anti-IRS1 (phospho S616) antibody 图像

  • All lanes : Anti-IRS1 (phospho S616) antibody (ab58479) at 1/500 dilution

    Lane 1 : HUVEC (Human umbilical vein endothelial cells) treated with insulin 0.01U/mL30' cell lysate
    Lane 2 : HUVEC (Human umbilical vein endothelial cells) treated with insulin 0.01U/mL30' cell lysate with Phospho peptide


    Predicted band size : 131 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab58479 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab58479 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.

Anti-IRS1 (phospho S616) antibody (ab58479)参考文献

ab58479 has not yet been referenced specifically in any publications.

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