Anti-IRS1 (phospho S616)抗体(ab4776)

概述

  • 产品名称Anti-IRS1 (phospho S616)抗体
    参阅全部 IRS1 一抗
  • 描述
    兔多克隆抗体to IRS1 (phospho S616)
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from a region of IRS 1 that contains serine 616. The sequence is conserved in human, mouse, and rat.

  • 阳性对照
    • CHO cell line transfected with a vector encoding the human insulin receptor (CHO-T) and transiently transfected with a vector encoding IRS 1, stimulated with a phorbol ester, TPA.

性能

应用

Our Abpromise guarantee covers the use of ab4776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 165 kDa.

靶标

  • 功能May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • 疾病相关Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • 序列相似性Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 翻译后修饰Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Anti-IRS1 (phospho S616) antibody 图像



  • Predicted band size : 165 kDa


    Extracts prepared from CHO-T cells mock transfected (Mock – lanes 1, 4), transiently transfected with wild-type human IRS 1 (WT – lanes 2, 5), and transiently transfected with mutant human IRS 1 (S616A – lanes 3, 6) and treated with TPA were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. After blocking non-specific sites with a 5% BSA-TBST buffer overnight at 4oC, the membranes were incubated with 0.50 µg/mL ab4776 antibody (lanes 1-3), or an anti-IRS 1 pan antibody (lanes 4-6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase. Signals were detected using the Tropix WesternStar method. The data show that the ab4776 antibody yields a signal on only the wild-type IRS 1 extract, not the mock or mutant IRS 1 extracts, thereby demonstrating the specificity of the antibody to this site. The data also show that the overall level of IRS 1 does not change between the wild-type and the mutant IRS 1 extracts (compare lanes 5 and 6).

Anti-IRS1 (phospho S616) antibody (ab4776)参考文献

This product has been referenced in:
  • Kawakami A  et al. Apolipoprotein CIII links hyperlipidemia with vascular endothelial cell dysfunction. Circulation 118:731-42 (2008). Read more (PubMed: 18663085) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry and patience. I contacted the originator of this antibody to see if they could provide clarification regarding this. Phospho-IRS-1 (Ser616) in human corresponds to Ser612 of mouse IRS-1. Also, the human, rat and mouse sequen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"