Anti-IRS1 (phospho S522)抗体(ab65745)

概述

  • 产品名称
    Anti-IRS1 (phospho S522)抗体
    参阅全部 IRS1 一抗
  • 描述
    羊多克隆抗体to IRS1 (phospho S522)
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide: CFRKRTHSPAGT conjugated to KLH, corresponding to amino acids 521-530 of Human IRS1

  • 阳性对照
    • Over-expressed IRS1 protein in extracts from CHO.IR.IRS1 with or without insulin treatment.

性能

应用

Our Abpromise guarantee covers the use of ab65745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 132 kDa.

靶标

  • 功能
    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • 疾病相关
    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • 序列相似性
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 翻译后修饰
    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

实验方案

文献

ab65745 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - other (Skeletal muscle, human)
Loading amount
100 µg
Specification
Skeletal muscle, human
Gel Running Conditions
Reduced Denaturing (5 %)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
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提交于 Aug 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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