概述

  • 产品名称Anti-IRS1 (phospho S307)抗体
    参阅全部 IRS1 一抗
  • 描述
    兔多克隆抗体to IRS1 (phospho S307)
  • 特异性Recognises a 180kDa band in 293 cells stimulated with anisomysin after serum starvation.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Pig
  • 免疫原

    Synthetic phospho peptide: GLTRRSRTESpITATSPASM conjugated to KLH, corresponding to amino acids 298-316 of Human IRS 1.

性能

应用

Our Abpromise guarantee covers the use of ab1194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).

靶标

  • 功能May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • 疾病相关Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • 序列相似性Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 翻译后修饰Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Anti-IRS1 (phospho S307) antibody 图像

  • All lanes : Anti-IRS1 (phospho S307) antibody (ab1194) at 1/250 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min.

    Lysates/proteins at 25 µg per lane.

    Secondary
    IRDye800 Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 131 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

Anti-IRS1 (phospho S307) antibody (ab1194)参考文献

This product has been referenced in:
  • Stuart CA  et al. Insulin resistance and muscle insulin receptor substrate-1 serine hyperphosphorylation. Physiol Rep 2:N/A (2014). Read more (PubMed: 25472611) »

See 1 Publication for this product

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